Evidence map›Paper›PMID 28676704›Full record

ArticleScientific reports2017

Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes.

Chin Siang Ong, Takuma Fukunishi, Huaitao Zhang, Chen Yu Huang, Andrew Nashed, Adriana Blazeski, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.

0numbers the graph read from it
0cells of the map it votes in
118citing papers in PubMed
12.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

118 citing papers in PubMed, 244 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Review
  19. Review
  20. Review

58 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Chin Siang OngDivision of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Takuma FukunishiDivision of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Huaitao ZhangDivision of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Chen Yu HuangDivision of Cardiology, Johns Hopkins Hospital, Baltimore, Maryland, USA.ORCID 0000-0002-9932-5651
Andrew NashedDivision of Cardiology, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Adriana BlazeskiDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Deborah DiSilvestreDivision of Cardiology, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Luca VricellaDivision of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland, USA.
John ConteDivision of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Leslie TungDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Gordon F TomaselliDivision of Cardiology, Johns Hopkins Hospital, Baltimore, Maryland, USA.
Narutoshi HibinoDivision of Cardiac Surgery, Johns Hopkins Hospital, Baltimore, Maryland, USA. nhibino1@jhmi.edu.
Johns Hopkins Hospital · USJohns Hopkins University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping. Cardiac tissue patches of all cell ratios beat spontaneously after 3D bioprinting. Patches exhibited ventricular-like action potential waveforms and uniform electrical conduction throughout the patch. Conduction velocities were higher and action potential durations were significantly longer in patches containing a lower percentage of FBs. Immunohistochemistry revealed staining for CM, FB and EC markers, with rudimentary CD31+ blood vessel formation. Immunofluorescence revealed the presence of Cx43, the main cardiac gap junction protein, localized to cell-cell borders. In vivo implantation suggests vascularization of 3D bioprinted cardiac patches with engraftment into native rat myocardium. This constitutes a significant step towards a new generation of stem cell-based treatment for heart failure.

Indexed as

Biocompatible MaterialsBioprintingInduced Pluripotent Stem CellsMyocytes, CardiacPrinting, Three-DimensionalTissue EngineeringTissue ScaffoldsAnimalsCell DifferentiationCells, CulturedElectrophysiological PhenomenaEndothelial CellsFibroblastsMyocardiumRatsSpheroids, CellularBiocompatible Materials

Identifiers

PMID28676704
PMCPMC5496874
OpenAlexW2729331518

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.