ArticleScientific reports2017
Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Bioluminescence-basedFrontiers in cellular and infection microbiology · 2026Article
- Comparative analysis of model antigen expression level driven by the microneme protein promoters in Eimeria tenella.Poultry science · 2025Article
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- Modes and mechanisms for the inheritance of mitochondria and plastids in pathogenic protists.PLoS pathogens · 2025Review
- Developing efficient strategies for localizing the enhanced yellow fluorescent protein subcellularly in transgenic Eimeria parasites.Scientific reports · 2024Article
- What Do We Know about Surface Proteins of Chicken ParasitesLife (Basel, Switzerland) · 2023Review
- Do All Coccidia Follow the Same Trafficking Rules?Life (Basel, Switzerland) · 2021Review
- Next-Generation Technologies and Systems Biology for the Design of Novel Vaccines Against Apicomplexan Parasites.Frontiers in veterinary science · 2021Review
- Effects of diclazuril on the expression of enolase in second-generation merozoites of Eimeria tenella.Poultry science · 2020Article
- Eimeria tenella Eimeria-specific protein that interacts with apical membrane antigen 1 (EtAMA1) is involved in host cell invasion.Parasites & vectors · 2020Article
- Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis.Parasites & vectors · 2020Article
- Towards Innovative Design and Application of RecombinantInfection and immunity · 2020Review
- The Growth ofFrontiers in cellular and infection microbiology · 2020Article
- Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella.Parasites & vectors · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Eimeria spp. are intracellular parasites that have a major impact on poultry. Effective live vaccines are available and the development of reverse genetic technologies has raised the prospect of using Eimeria spp. as recombinant vectors to express additional immunoprotective antigens. To study the ability of Eimeria to secrete foreign antigens or display them on the surface of the sporozoite, transiently transfected populations of E. tenella expressing the fluorescent protein mCherry, linked to endogenous signal peptide (SP) and glycophosphatidylinositol-anchor (GPI) sequences, were examined. The SP from microneme protein EtMIC2 (SP2) allowed efficient trafficking of mCherry to cytoplasmic vesicles and following the C-terminal addition of a GPI-anchor (from surface antigen EtSAG1) mCherry was expressed on the sporozoite surface. In stable transgenic populations, mCherry fused to SP2 was secreted into the sporocyst cavity of the oocysts and after excystation, secretion was detected in culture supernatants but not into the parasitophorous vacuole after invasion. When the GPI was incorporated, mCherry was observed on the sporozites surface and in the supernatant of invading sporozoites. The proven secretion and surface exposure of mCherry suggests that antigen fusions with SP2 and GPI of EtSAG1 may be promising candidates to examine induction of protective immunity against heterologous pathogens.
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