ArticleScientific reports2017
CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Notch Signaling in Insect Development: A Simple Pathway with Diverse Functions.International journal of molecular sciences · 2023Review
- Article
- From Aedes to Zeugodacus: a review of dipteran body coloration studies regarding evolutionary developmental biology, pest control, and species discovery.Current opinion in genetics & development · 2021Review
- Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes.PLoS biology · 2021Article
- Yellow-y Functions in Egg Melanization and Chorion Morphology of the Asian Tiger Mosquito,Frontiers in cell and developmental biology · 2021Article
- Article
- Specific Gene Disruption in the Major Livestock PestsG3 (Bethesda, Md.) · 2019Article
- CRISPR-induced double-strand breaks trigger recombination between homologous chromosome arms.Life science alliance · 2019Article
- Allele-specific genome editing using CRISPR-Cas9 is associated with loss of heterozygosity in diploid yeast.Nucleic acids research · 2019Article
- Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing.Nature communications · 2018Article
- Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?International journal of environmental research and public health · 2017Review
- Highly efficient DNA-free gene disruption in the agricultural pest Ceratitis capitata by CRISPR-Cas9 ribonucleoprotein complexes.Scientific reports · 2017Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The classic brown body (bwb) mutation in the housefly Musca domestica impairs normal melanization of the adult cuticle. In Drosophila melanogaster, a reminiscent pigmentation defect results from mutations in the yellow gene encoding dopachrome conversion enzyme (DCE). Here, we demonstrate that the bwb locus structurally and functionally represents the yellow ortholog of Musca domestica, MdY. In bwb Musca strains, we identified two mutant MdY alleles that contain lesions predicted to result in premature truncation of the MdY open reading frame. We targeted wildtype MdY by CRISPR-Cas9 RNPs and generated new mutant alleles that fail to complement existing MdY alleles, genetically confirming that MdY is the bwb locus. We further found evidence for Cas9-mediated interchromosomal recombination between wildtype and mutant bwb alleles. Our work resolves the molecular identity of the classic bwb mutation in Musca domestica and establishes the feasibility of Cas9-mediated genome editing in the Musca model.
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