Evidence map›Paper›PMID 28674954›Full record

ArticleEnvironmental science and pollution research international2017

Biosynthesis of staphylococcal enterotoxin A by genetic engineering technology and determination of staphylococcal enterotoxin A in water by HPLC-ESI-TOF.

Hong-Na Li, Fei Yuan, Yun-Jing Luo, Jian-Feng Wang, Chuan-Bin Zhang, Wei-E Zhou, Zhi-Qin Ren, Wen-Jie Wu, Feng Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Environmental science and pollution research international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Hong-Na LiInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Fei YuanInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Yun-Jing LuoCollege of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China.
Jian-Feng WangInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Chuan-Bin ZhangInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Wei-E ZhouInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Zhi-Qin RenInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Wen-Jie WuInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Feng ZhangInstitute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China. fengzhang@126.com.
Chinese Academy of Inspection and Quarantine · CNBeijing University of Technology · CN

Funding

Basic Research Fund for Central Public Interest Institutes 2017JK016Public Welfare Fund of Inspection and Quarantine of China 201510038Science and Technology Project of AQSIQ of China 2014IK084
6 · The paper itself

Abstract

Staphylococcal enterotoxin A (SEA) was the major virulence factor of Staphylococcus aureus and a biomarker of S. aureus. To establish a fast, low cost, high accuracy, reliable, and simple method for detecting S. aureus, SEA was analyzed by HPLC-ESI-TOF. SEA was not yet commercially available in universal, so SEA was prepared before it was analyzed by HPLC-ESI-TOF. The result showed that high purified SEA was successfully prepared and SEA has normal distribution in mass spectra. A large amount of recombinant SEA (rSEA) was obtained by engineering technology and was purified by Ni affinity chromatography column, and the expression and purity of rSEA and SEA were analyzed by SDS-PAGE. The factors effected on ionization of SEA were studied, and the qualitative analysis of SEA by HPLC-ESI-TOF. The result showed that large amount of SEs expressed within a short time at 28 °C or thereabouts, and there was no impurity bands in electrophorogram after rSEA was purified by Ni affinity chromatography column. In addition, the SEA which had homologous AA sequence with wild SEA was made by rSEA. The retention of SEA in column and ionization of SEA in ESI-TOF were studied for qualitative analysis of S. aureus. The result showed that the content of formic acid in mobile phase was an important factor for ionization of SEs in ESI-TOF. And the result provided theoretical foundation for qualitative detection of S. aureus. [SEs + nH

Indexed as

Chemistry Techniques, AnalyticalChromatography, AffinityChromatography, High Pressure LiquidCloning, MolecularEnterotoxinsGenetic EngineeringMass SpectrometryRecombinant ProteinsStaphylococcus aureusWaterenterotoxin A, StaphylococcalEnterotoxinsRecombinant ProteinsWaterBiosynthesisHigh-performance liquid chromatographyQualitative analysisStaphylococcal enterotoxin ATime of flight

Identifiers

PMID28674954
OpenAlexW2730412992

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.