Evidence map›Paper›PMID 28674809›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells.

Amelia J Blake, Danielle S Finger, Victoria L Hardy, Elizabeth T Ables

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Article
  3. SR Protein Kinase is expressed inmicroPublication biology · 2025
    Article
  4. Life science alliance · 2023
    Article
  5. Article
  6. Article
  7. TheFrontiers in physiology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Amelia J BlakeEast Carolina University, 1001 E. 10th Street, Mailstop 551, Greenville, NC, 27858, USA.
Danielle S FingerEast Carolina University, 1001 E. 10th Street, Mailstop 551, Greenville, NC, 27858, USA.
Victoria L HardyEast Carolina University, 1001 E. 10th Street, Mailstop 551, Greenville, NC, 27858, USA.
Elizabeth T AblesEast Carolina University, 1001 E. 10th Street, Mailstop 551, Greenville, NC, 27858, USA. ablese@ecu.edu.
East Carolina University · US

Funding

Molecular mechanisms of oocyte development in DrosophilaR15GM117502 · NIGMS · EAST CAROLINA UNIVERSITY · PI ABLES, ELIZABETH TWEEDIE · 2016 to 2023
$1.4M
NIGMS NIH HHS R15 GM117502
6 · The paper itself

Abstract

Elucidating the full repertoire of molecular mechanisms that promote stem cell maintenance requires sophisticated techniques for identifying and characterizing gene function in stem cells in their native environment. Ovarian germline stem cells in the fruit fly, Drosophila melanogaster, are an ideal model to study the complex molecular mechanisms driving stem cell function in vivo. A variety of new genetic tools make RNAi a useful complement to traditional genetic mutants for the investigation of the molecular mechanisms guiding ovarian germline stem cell function. Here, we provide a detailed guide for using targeted RNAi knockdown for the discovery of gene function in ovarian germline stem cells and their progeny.

Indexed as

Gene Expression Regulation, DevelopmentalRNA InterferenceAnimalsDrosophilaDrosophila ProteinsFemaleGerm CellsMicroscopy, ConfocalOogenesisOvaryPhenotypeDrosophila ProteinsDrosophilaGerm cellGermlineOogenesisOvaryStem cell

Identifiers

PMID28674809
PMCPMC5798201
OpenAlexW2727701120

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.