ArticleAnalytical methods : advancing methods and applications2017
Analysis of Stable Isotope Assisted Metabolomics Data Acquired by High Resolution Mass Spectrometry.
Article in Analytical methods : advancing methods and applications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Pyruvate kinase splice variants in fibroblasts influence cardiac remodeling after myocardial infarction in male mice.Journal of molecular and cellular cardiology · 2025Article
- Phase partitioning of the neutrophil oxidative burst is coordinated by accessory pathways of glucose metabolism and mitochondrial activity.The Journal of biological chemistry · 2025Article
- Tracing priming effects in palsa peat carbon dynamics using a stable isotope-assisted metabolomics approach.Frontiers in molecular biosciences · 2025Article
- Untangling the web of glioblastoma treatment resistance using a multi-omic and multidisciplinary approach.The American journal of the medical sciences · 2023Review
- Article
- Glutamine uptake and catabolism is required for myofibroblast formation and persistence.Journal of molecular and cellular cardiology · 2022Article
- Differential metabolic requirement governed by transcription factor c-Maf dictates innate γδT17 effector functionality in mice and humans.Science advances · 2022Article
- Bioengineered Models to Study Microenvironmental Regulation of Glioblastoma Metabolism.Journal of neuropathology and experimental neurology · 2021Review
- Cell cycle induction in human cardiomyocytes is dependent on biosynthetic pathway activation.Redox biology · 2021Article
- Evaluation of disease staging and chemotherapeutic response in non-small cell lung cancer from patient tumor-derived metabolomic data.Lung cancer (Amsterdam, Netherlands) · 2021Article
- Considerations for using isolated cell systems to understand cardiac metabolism and biology.Journal of molecular and cellular cardiology · 2021Review
- Transcription factor c-Maf is a checkpoint that programs macrophages in lung cancer.The Journal of clinical investigation · 2020Article
- Loss ofCancers · 2020Article
- Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells.Cancer cell international · 2020Article
- Integration of flux measurements and pharmacological controls to optimize stable isotope-resolved metabolomics workflows and interpretation.Scientific reports · 2019Article
- Article
- Profiling of Polar Metabolites in Mouse Feces Using Four Analytical Platforms to Study the Effects Of Cathelicidin-Related Antimicrobial Peptide in Alcoholic Liver Disease.Journal of proteome research · 2019Article
- Cardiac mesenchymal cells from diabetic mice are ineffective for cell therapy-mediated myocardial repair.Basic research in cardiology · 2018Article
- Analysis of stable isotope assisted metabolomics data acquired by GC-MS.Analytica chimica acta · 2017Article
- Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes.The Biochemical journal · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Stable isotope assisted metabolomics (SIAM) uses stable isotope tracers to support studies of biochemical mechanisms. We report a suite of data analysis algorithms for automatic analysis of SIAM data acquired on a high resolution mass spectrometer. To increase the accuracy of isotopologue assignment, metabolites detected in the unlabeled samples were used as reference metabolites to generate possible isotopologue candidates for analysis of peaks detected in the labeled samples. An iterative linear regression model was developed to deconvolute the overlapping isotopic peaks of isotopologues present in a full MS spectrum, where the threshold for the weight factor was determined by a simulation study assuming different levels of Gaussian white noise contamination. A normalization method enabling isotope ratio-based normalization was implemented to study the difference of isotopologue abundance distribution between sample groups. The developed method can analyze SIAM data acquired by direct infusion MS and LC-MS, and can handle metabolite tracers containing different tracer elements. Analysis of SIAM data acquired from mixtures of known compounds showed that the developed algorithms accurately identify metabolites and quantify stable isotope enrichment. Application of SIAM data acquired from a biological study further demonstrated the effectiveness and accuracy of the developed method for analysis of complex samples.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.