ArticleScientific reports2017
PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Systematic production of human kidney organoids for transplantation in porcine kidneys during ex vivo machine perfusion.Nature biomedical engineering · 2026Article
- Expansion of human pluripotent stem cell-induced nephron progenitor cells (iNPCs) and the generation of nephron organoids from iNPCs.Nature protocols · 2026Review
- Caspase 9-induced apoptosis enables efficient fetal cell ablation and disease modeling.Nature communications · 2025Article
- Comparative analysis ofbioRxiv : the preprint server for biology · 2025Article
- Experimental models for preclinical research in kidney disease.Zoological research · 2024Review
- Advancements in therapeutic development: kidney organoids and organs on a chip.Kidney international · 2024Review
- Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition.Developmental cell · 2024Article
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- Harnessing mechanobiology for kidney organoid research.Frontiers in cell and developmental biology · 2023Review
- Engraftment of Kidney Organoids In Vivo.Current transplantation reports · 2023Review
- Kidney organoids: a pioneering model for kidney diseases.Translational research : the journal of laboratory and clinical medicine · 2022Review
- DevKidCC allows for robust classification and direct comparisons of kidney organoid datasets.Genome medicine · 2022Article
- Article
- Impaired NEPHRIN localization in kidney organoids derived from nephrotic patient iPS cells.Scientific reports · 2021Article
- Renal proximal tubular epithelial cells: review of isolation, characterization, and culturing techniques.Molecular biology reports · 2020Review
- Stem cell-derived kidney organoids: engineering the vasculature.Cellular and molecular life sciences : CMLS · 2020Review
- The Utility of Human Kidney Organoids in Modeling Kidney Disease.Seminars in nephrology · 2020Review
- Human kidney organoids: progress and remaining challenges.Nature reviews. Nephrology · 2019Review
- Molecular characterization of nephron progenitors and their early epithelial derivative structures in the nephrogenic zone of the canine fetal kidney.European journal of histochemistry : EJH · 2019Article
- Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors.Stem cell reports · 2019Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The kidney is formed by reciprocal interactions between the nephron progenitor and the ureteric bud, the former of which gives rise to the epithelia of nephrons consisting of glomeruli and renal tubules. The transcription factor PAX2 is essential for this mesenchymal-to-epithelial transition of nephron progenitors, as well as ureteric bud lineage development, in mice. PAX2 mutations in humans cause renal coloboma syndrome. We previously reported the induction of nephron progenitors and three-dimensional nephron structures from human induced pluripotent stem (iPS) cells. Here we generate iPS cells lacking PAX2, and address the role of PAX2 in our in vitro induction protocol. While PAX2-null human nephron progenitors were properly formed, they unexpectedly became epithelialised to form glomeruli and renal tubules. However, the mutant glomerular parietal epithelial cells failed to transit to the squamous morphology, retaining the shape and markers of columnar epithelia. Therefore, PAX2 is dispensable for mesenchymal-to-epithelial transition of nephron progenitors, but is required for morphological development of glomerular parietal epithelial cells, during nephron formation from human iPS cells in vitro.
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