ArticleNature communications2017
Insulated transcriptional elements enable precise design of genetic circuits.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Hfq-dependent artificial regulatory small RNAs for targeted gene expression control in the nitrogen-fixingSynthetic and systems biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- A unified computational framework for quantitative design and optimization of transcriptional regulation across bacterial species.Nucleic acids research · 2026Article
- Extrusion-modulated DnaA activity oscillations coordinate DNA replication with biomass growth.eLife · 2025Article
- Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable.ACS synthetic biology · 2025Review
- De novo design of insulated cis-regulatory elements based on deep learning-predicted fitness landscape.Nucleic acids research · 2025Article
- Expanding the σ54-dependent transcription process with orthogonal designs.Nucleic acids research · 2025Article
- Developing Quorum Sensing-Based Collaborative Dynamic Control System in Halomonas TD01.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Quantitative Characterization of Gene Regulatory Circuits Associated With Fungal Secondary Metabolism to Discover Novel Natural Products.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Assembly of functional microbial ecosystems: from molecular circuits to communities.FEMS microbiology reviews · 2024Review
- Energy Aware Technology Mapping of Genetic Logic Circuits.ACS synthetic biology · 2024Article
- Energy Aware Technology Mapping of Genetic Logic Circuits.bioRxiv : the preprint server for biology · 2024Article
- Article
- Emergent digital bio-computation through spatial diffusion and engineered bacteria.Nature communications · 2024Article
- Functional predictability of universal gene circuits in diverse microbial hosts.Quantitative biology (Beijing, China) · 2024Article
- The dynamic-process characterization and prediction of synthetic gene circuits by dynamic delay model.iScience · 2024Article
- Accelerating Genetic Sensor Development, Scale-up, and Deployment Using Synthetic Biology.Biodesign research · 2024Article
- Customizing cellular signal processing by synthetic multi-level regulatory circuits.Nature communications · 2023Review
- Structure of the transcription open complex of distinct σNature communications · 2023Article
- Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene Biosynthesis.ACS synthetic biology · 2023Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rational engineering of biological systems is often complicated by the complex but unwanted interactions between cellular components at multiple levels. Here we address this issue at the level of prokaryotic transcription by insulating minimal promoters and operators to prevent their interaction and enable the biophysical modeling of synthetic transcription without free parameters. This approach allows genetic circuit design with extraordinary precision and diversity, and consequently simplifies the design-build-test-learn cycle of circuit engineering to a mix-and-match workflow. As a demonstration, combinatorial promoters encoding NOT-gate functions were designed from scratch with mean errors of <1.5-fold and a success rate of >96% using our insulated transcription elements. Furthermore, four-node transcriptional networks with incoherent feed-forward loops that execute stripe-forming functions were obtained without any trial-and-error work. This insulation-based engineering strategy improves the resolution of genetic circuit technology and provides a simple approach for designing genetic circuits for systems and synthetic biology.Unwanted interactions between cellular components can complicate rational engineering of biological systems. Here the authors design insulated minimal promoters and operators that enable biophysical modeling of bacterial transcription without free parameters for precise circuit design.
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