ArticleProceedings of the National Academy of Sciences of the United States of America2017
Changes in E-cadherin rigidity sensing regulate cell adhesion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 89 citations in OpenAlex.
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- Extracellular Matrix Viscoelasticity Regulates Mammary Branching Morphogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cadherin dynamics and cortical tension in remodeling cell-cell adhesion during EMT.Biophysical journal · 2025Article
- ETVs dictate hPSC differentiation by tuning biophysical properties.Nature communications · 2025Article
- Cell Architecture and Dynamics of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) on Hydrogels with Spatially Patterned Laminin and N-Cadherin.ACS applied materials & interfaces · 2025Article
- Spatial heterogeneity in tumor adhesion qualifies collective cell invasion.Biophysical journal · 2024Article
- Field Guide to Traction Force Microscopy.Cellular and molecular bioengineering · 2024Review
- E-cadherin biomaterials reprogram collective cell migration and cell cycling by forcing homeostatic conditions.Cell reports · 2024Article
- Roles of the Dbl family of RhoGEFs in mechanotransduction - a review.Frontiers in cell and developmental biology · 2024Review
- Building Biomaterials to Mimic 3D Cell-Cell Junctions.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Adhesome Receptor Clustering is Accompanied by the Colocalization of the Associated Genes in the Cell Nucleus.bioRxiv : the preprint server for biology · 2023Article
- Vinculin is essential for sustaining normal levels of endogenous forces at cell-cell contacts.Biophysical journal · 2023Article
- Cell-cell interactions during the formation of primordial follicles in humans.Life science alliance · 2023Article
- Push-pull mechanics of E-cadherin ectodomains in biomimetic adhesions.Biophysical journal · 2023Article
- Engineering tools for quantifying and manipulating forces in epithelia.Biophysics reviews · 2023Review
- Mechanisms of primordial follicle activation and new pregnancy opportunity for premature ovarian failure patients.Frontiers in physiology · 2023Review
- Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylation.Nature communications · 2022Article
- Assessing the roles of collagen fiber morphology and matrix stiffness on ovarian cancer cell migration dynamics using multiphoton fabricated orthogonal image-based models.Acta biomaterialia · 2022Article
- Mechanical transmission enables EMT cancer cells to drive epithelial cancer cell migration to guide tumor spheroid disaggregation.Science China. Life sciences · 2022Article
- Intrinsic cell rheology drives junction maturation.Nature communications · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mechanical cues are sensed and transduced by cell adhesion complexes to regulate diverse cell behaviors. Extracellular matrix (ECM) rigidity sensing by integrin adhesions has been well studied, but rigidity sensing by cadherins during cell adhesion is largely unexplored. Using mechanically tunable polyacrylamide (PA) gels functionalized with the extracellular domain of E-cadherin (Ecad-Fc), we showed that E-cadherin-dependent epithelial cell adhesion was sensitive to changes in PA gel elastic modulus that produced striking differences in cell morphology, actin organization, and membrane dynamics. Traction force microscopy (TFM) revealed that cells produced the greatest tractions at the cell periphery, where distinct types of actin-based membrane protrusions formed. Cells responded to substrate rigidity by reorganizing the distribution and size of high-traction-stress regions at the cell periphery. Differences in adhesion and protrusion dynamics were mediated by balancing the activities of specific signaling molecules. Cell adhesion to a 30-kPa Ecad-Fc PA gel required Cdc42- and formin-dependent filopodia formation, whereas adhesion to a 60-kPa Ecad-Fc PA gel induced Arp2/3-dependent lamellipodial protrusions. A quantitative 3D cell-cell adhesion assay and live cell imaging of cell-cell contact formation revealed that inhibition of Cdc42, formin, and Arp2/3 activities blocked the initiation, but not the maintenance of established cell-cell adhesions. These results indicate that the same signaling molecules activated by E-cadherin rigidity sensing on PA gels contribute to actin organization and membrane dynamics during cell-cell adhesion. We hypothesize that a transition in the stiffness of E-cadherin homotypic interactions regulates actin and membrane dynamics during initial stages of cell-cell adhesion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.