Evidence map›Paper›PMID 28674014›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2017

E-cadherin and LGN align epithelial cell divisions with tissue tension independently of cell shape.

Kevin C Hart, Jiongyi Tan, Kathleen A Siemers, Joo Yong Sim, Beth L Pruitt, W James Nelson, Martijn Gloerich

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 104 citations in OpenAlex.

  1. Article
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  10. Tension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane.Proceedings of the National Academy of Sciences of the United States of America · 2022
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  11. Review
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  15. Spindle reorientation in response to mechanical stress is an emergent property of the spindle positioning mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2022
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  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Kevin C HartDepartment of Biology, Stanford University, Stanford, CA 94305.
Jiongyi TanBiophysics Program, Stanford University, Stanford, CA 94305.
Kathleen A SiemersDepartment of Biology, Stanford University, Stanford, CA 94305.
Joo Yong SimDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305.
Beth L PruittDepartment of Mechanical Engineering, Stanford University, Stanford, CA 94305.
W James NelsonDepartment of Biology, Stanford University, Stanford, CA 94305.
Martijn GloerichDepartment of Biology, Stanford University, Stanford, CA 94305; m.gloerich@umcutrecht.nl.
Stanford University · USCardiovascular Institute of the South · US

Funding

Supplement to Enhance Wellness and Resiliency in the Graduate EnvironmentT32GM007276 · NIGMS · STANFORD UNIVERSITY · PI MORRISON, ASHBY J. · 1985 to 2023
$32.9M
Molecular Biophysics Training Program at StanfordT32GM008294 · NIGMS · STANFORD UNIVERSITY · PI HUANG, KERWYN C. · 1989 to 2019
$7.4M
Cell-Cell Junctions and Epithelial HomeostasisR35GM118064 · NIGMS · STANFORD UNIVERSITY · PI STEARNS, TIM · 2016 to 2018
$2.6M
NIGMS NIH HHS R35 GM118064NIGMS NIH HHS T32 GM007276NIGMS NIH HHS T32 GM008294
6 · The paper itself

Abstract

Tissue morphogenesis requires the coordinated regulation of cellular behavior, which includes the orientation of cell division that defines the position of daughter cells in the tissue. Cell division orientation is instructed by biochemical and mechanical signals from the local tissue environment, but how those signals control mitotic spindle orientation is not fully understood. Here, we tested how mechanical tension across an epithelial monolayer is sensed to orient cell divisions. Tension across Madin-Darby canine kidney cell monolayers was increased by a low level of uniaxial stretch, which oriented cell divisions with the stretch axis irrespective of the orientation of the cell long axis. We demonstrate that stretch-induced division orientation required mechanotransduction through E-cadherin cell-cell adhesions. Increased tension on the E-cadherin complex promoted the junctional recruitment of the protein LGN, a core component of the spindle orientation machinery that binds the cytosolic tail of E-cadherin. Consequently, uniaxial stretch triggered a polarized cortical distribution of LGN. Selective disruption of

Indexed as

AnimalsCadherinsCell AdhesionCell DivisionCell ShapeDogsEpithelial CellsGreen Fluorescent ProteinsIntracellular Signaling Peptides and ProteinsMadin Darby Canine Kidney CellsMechanotransduction, CellularSpindle ApparatusStress, MechanicalTubulinCadherinsGreen Fluorescent ProteinsIntracellular Signaling Peptides and ProteinsTubulincell–cell adhesioncell division orientationmechanotransductionmitotic spindle

Identifiers

PMID28674014
PMCPMC5530667
OpenAlexW2724850827

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.