ArticleProceedings of the National Academy of Sciences of the United States of America2017
Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
75 citing papers in PubMed, 151 citations in OpenAlex.
- Interleukin-4 Supplementation During In Vitro Culture Modulates the Expression of Stress- and Immune-Related Genes During Porcine Blastocyst Development.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Activating a patient-relevant pathogenic variant of a genetically defined heart disease in a humanized pig model.Lab animal · 2026Article
- Overcoming Barriers in Porcine SCNT: A Comprehensive Review of Developmental Challenges and Innovations.International journal of molecular sciences · 2026Review
- Effects of Fluorescence-Activated Cell Sorting on Boar Sperm Motility Subpopulations and In Vitro Embryo Development.Molecular reproduction and development · 2026Article
- Gene editing of the GJB2 locus in porcine embryos using CRISPR/Cas9 and cytosine base editors: toward a model of congenital deafness.Scientific reports · 2026Article
- The Critical Functions of FGF2, LIF and IGF1 in the Improvement of In Vitro Embryo Production.Biomolecules · 2026Review
- BDNF Promotes In Vitro Maturation of Sheep Oocytes by Alleviating Oxidative Stress and Endoplasmic Reticulum Stress.Antioxidants (Basel, Switzerland) · 2026Article
- Enhancing the specificity of gene editing outcomes by using Cas9 variants in porcine embryos.Journal of animal science · 2026Article
- Advances in pig embryo production for cryopreservation, transfer and genome editing.The Journal of reproduction and development · 2026Review
- Cross-Species Insights intoAntioxidants (Basel, Switzerland) · 2025Article
- Establishment of a Single-Oocyte Culture System for Pigs and Its Validation Using Curcumin as a Model Antioxidant for Oocyte Maturation.Animals : an open access journal from MDPI · 2025Article
- Decoding the Molecular Landscape of Prepubertal Oocyte Maturation: GTPBP4 as a Key Driver of In Vitro Developmental Competence.Cell proliferation · 2025Article
- Cytokine supplementation influences bovine embryo transcriptome during the preimplantation period.Reproduction & fertility · 2025Article
- Expression of tripartite motif family-like protein 1 and 2 in early conceptus development and placentation in the pig.Reproduction & fertility · 2025Article
- DPImpute: A Genotype Imputation Framework for Ultra-Low Coverage Whole-Genome Sequencing and its Application in Genomic Selection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Gene Editing for Enhanced Swine Production: Current Advances and Prospects.Animals : an open access journal from MDPI · 2025Review
- Stage-dependent changes in culture medium osmolality promote porcine oocyte maturationFrontiers in cell and developmental biology · 2025Article
- A porcine model of Fanconi anemia.PloS one · 2025Article
- Dynamics and necessity of SIRT1 for maternal-zygotic transition.Scientific reports · 2024Article
- Development and Initial Characterization of Pigs withbioRxiv : the preprint server for biology · 2024Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to the blastocyst stage, and give rise to live young than their in vivo-produced counterparts, particularly if they are derived from immature females. Here we show that a chemically defined maturation medium supplemented with three cytokines (FGF2, LIF, and IGF1) in combination, so-called "FLI medium," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovaries and provides a twofold increase in the efficiency of blastocyst production after in vitro fertilization. Transfer of such blastocysts to recipient females doubles mean litter size to about nine piglets per litter. Maturation of oocytes in FLI medium, therefore, effectively provides a fourfold increase in piglets born per oocyte collected. As they progress in culture, the FLI-matured cumulus-oocyte complexes display distinctly different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte within. These events likely underpin the improvement in oocyte quality achieved by using the FLI medium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.