ArticleNature chemical biology2017
A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system.
Article in Nature chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- High-throughput monitoring of in vitro enzymatic reactions using probe electrospray ionization-mass spectrometry.Analytical and bioanalytical chemistry · 2026Article
- Ultra-rapid and high-titer biomanufacturing of trehalose 6-phosphate by an in vitro synthetic biology platform.Bioresources and bioprocessing · 2026Article
- Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Towards Synthetic Cells with Self-Producing Energy.ChemPlusChem · 2024Review
- Cell-Free Protein Expression by a Reconstituted Transcription-Translation System Energized by Sugar Catabolism.Molecules (Basel, Switzerland) · 2024Article
- ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA.Nature communications · 2024Article
- Cell-Free Production and Regeneration of Cofactors.Advances in biochemical engineering/biotechnology · 2023Article
- Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes.Advances in biochemical engineering/biotechnology · 2023Article
- Toward bioproduction of oxo chemicals from CBiotechnology for biofuels and bioproducts · 2022Review
- Engineering Embden-Meyerhof-Parnas Glycolysis to Generate Noncanonical Reducing Power.ACS catalysis · 2022Article
- Build-a-Cell: Engineering a Synthetic Cell Community.Life (Basel, Switzerland) · 2021Article
- Cell-Free Total Biosynthesis of Plant Terpene Natural Products using an Orthogonal Cofactor Regeneration System.ACS catalysis · 2021Article
- Isobutanol production freed from biological limits using synthetic biochemistry.Nature communications · 2020Article
- A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds.Metabolic engineering communications · 2020Article
- MEMO: A Method for Computing Metabolic Modules for Cell-Free Production Systems.ACS synthetic biology · 2020Article
- A critical comparison of cellular and cell-free bioproduction systems.Current opinion in biotechnology · 2019Review
- Structural organization of biocatalytic systems: the next dimension of synthetic metabolism.Emerging topics in life sciences · 2019Article
- A synthetic metabolic network for physicochemical homeostasis.Nature communications · 2019Article
- A cell-free platform for the prenylation of natural products and application to cannabinoid production.Nature communications · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic biochemistry seeks to engineer complex metabolic pathways for chemical conversions outside the constraints of the cell. Establishment of effective and flexible cell-free systems requires the development of simple systems to replace the intricate regulatory mechanisms that exist in cells for maintaining high-energy cofactor balance. Here we describe a simple rheostat that regulates ATP levels by controlling the flow down either an ATP-generating or non-ATP-generating pathway according to the free-phosphate concentration. We implemented this concept for the production of isobutanol from glucose. The rheostat maintains adequate ATP concentrations even in the presence of ATPase contamination. The final system including the rheostat produced 24.1 ± 1.8 g/L of isobutanol from glucose in 91% theoretical yield with an initial productivity of 1.3 g/L/h. The molecular rheostat concept can be used in the design of continuously operating, self-sustaining synthetic biochemistry systems.
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Registered trials
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