Evidence map›Paper›PMID 28671653›Full record

ArticleJournal of visualized experiments : JoVE2017

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N 6-threonylcarbamoyladenosine TcdA Function.

Francisco J Fernández, Sara Gómez, Sergio Navas-Yuste, Miguel López-Estepa, M Cristina Vega

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Francisco J FernándezDepartment of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC); Department of Immunology, Complutense University School of Medicine; Abvance Biotech srl.
Sara GómezDepartment of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC).
Sergio Navas-YusteDepartment of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC).
Miguel López-EstepaDepartment of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC).
M Cristina VegaDepartment of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC); cvega@cib.csic.es.
Consejo Superior de Investigaciones Científicas · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We demonstrate methods for the expression and purification of tRNA(UUU) in Escherichia coli and the analysis by gel retardation assays of the binding of tRNA(UUU) to TcdA, an N

Indexed as

AdenosineElectrophoresis, Agar GelModels, MolecularNucleic Acid ConformationProtein BindingRNA, TransferAdenosineN(6)-(N-threonylcarbonyl)adenosineRNA, Transfer

Identifiers

PMID28671653
PMCPMC5608462
OpenAlexW2649508310

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.