ArticleScientific reports2017
Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 50 citations in OpenAlex.
- Plasmonic Metal-Phenolic Network Nanoprobes for Multiplex Dual-Mode Immunophenotyping.Nano letters · 2025Article
- Black Silicon Surface-Enhanced Raman Spectroscopy Biosensors: Current Advances and Prospects.Biosensors · 2024Review
- Advancing SERS as a quantitative technique: challenges, considerations, and correlative approaches to aid validation.Nano convergence · 2024Review
- From Raman to SESORRS: moving deeper into cancer detection and treatment monitoring.Chemical communications (Cambridge, England) · 2021Review
- Article
- Novel Methods and Approaches for Safety Evaluation of Nanoparticle Formulations: A Focus TowardsFrontiers in pharmacology · 2021Review
- Nanoneedle-Based Materials for Intracellular Studies.Advances in experimental medicine and biology · 2021Article
- Introduction to Infrared and Raman-Based Biomedical Molecular Imaging and Comparison with Other Modalities.Molecules (Basel, Switzerland) · 2020Review
- Distribution modeling of nanoparticles for brachytherapy of human eye tumor.EJNMMI physics · 2020Article
- Label-free plasmonic nanostar probes to illuminateChemical science · 2019Article
- Revealing membrane alteration in cellsoverexpressing CA IX and EGFR by Surface-Enhanced Raman Scattering.Scientific reports · 2019Article
- Review
- Nanoparticles for super-resolution microscopy and single-molecule tracking.Nature methods · 2018Review
- Comparison of Gold Nanoparticle and Fluorophore-conjugated Antibodies for Labeling Epitopes in Permeabilized Cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.