ArticleBMC microbiology2017
Soluble factors from biofilm of Candida albicans and Staphylococcus aureus promote cell death and inflammatory response.
Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Candida albicans hyphae modulate Staphylococcus aureus cell-free supernatant during dual biofilm growth to drive molecular signatures of oral dysplasia.Medical microbiology and immunology · 2026Article
- Cytokine and chemokine dysregulation by microbial biofilm components: mechanisms and clinical implications.Archives of microbiology · 2026Review
- Immune dysfunction during S. aureus biofilm-associated implant infections: opportunities for novel therapeutic strategies.NPJ biofilms and microbiomes · 2025Review
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- A customizable and defined medium supporting culturing ofApplied and environmental microbiology · 2024Article
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- Antifungal Activity and Biocompatibility of α-AgVOFrontiers in bioengineering and biotechnology · 2022Article
- Marine Fungal Cerebroside Flavuside B Protects HaCaT Keratinocytes againstMarine drugs · 2021Article
- Metabolic Adaptations DuringFrontiers in immunology · 2021Review
- Proto-Oncogenes and Cell Cycle Gene Expression in Normal and Neoplastic Oral Epithelial Cells Stimulated With Soluble Factors From Single and Dual Biofilms ofFrontiers in cellular and infection microbiology · 2021Article
- The Immune Functions of Keratinocytes in Skin Wound Healing.International journal of molecular sciences · 2020Review
- Biofilm growth and IL-8 & TNF-α-inducing properties of Candida albicans in the presence of oral gram-positive and gram-negative bacteria.BMC microbiology · 2020Article
- Article
- How Biofilm Growth AffectsFrontiers in microbiology · 2020Review
- Review
- [Research progress on interactions between Candida albicans and common oral pathogens].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2019Review
- Article
- Review
- Nonthermal Plasma Jet Treatment Negatively Affects the Viability and Structure of Candida albicans SC5314 Biofilms.Applied and environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe objective of this study was to better understand the effects of soluble factors from biofilm of single- and mixed-species Candida albicans (C. albicans) and methicillin-sensitive Staphylococcus aureus (MSSA) cultures after 36 h in culture on keratinocytes (NOK-si and HaCaT) and macrophages (J774A.1). Soluble factors from biofilms of C. albicans and MSSA were collected and incubated with keratinocytes and macrophages, which were subsequently evaluated by cell viability assays (MTT). Lactate dehydrogenase (LDH) enzyme release was measured to assess cell membrane damage to keratinocytes. Cells were analysed by brightfield microscopy after 2 and 24 h of exposure to the soluble factors from biofilm. Cell death was detected by labelling apoptotic cells with annexin V and necrotic cells with propidium iodide (PI) and was visualized via fluorescence microscopy. Soluble factors from biofilm were incubated with J774A.1 cells for 24 h; the subsequent production of NO and the cytokines IL-6 and TNF-α was measured by ELISA.
resultsThe cell viability assays showed that the soluble factors of single-species C. albicans cultures were as toxic as the soluble factors from biofilm of mixed cultures, whereas the soluble factors of MSSA cultures were less toxic than those of C. albicans or mixed cultures. The soluble factors from biofilm of mixed cultures were the most toxic to the NOK-si and HaCaT cells, as confirmed by analyses of PI labelling and cell morphology. Soluble factors from biofilm of single-species MSSA and mixed-species cultures induced the production of IL-6, NO and TNF-α by J744A.1 macrophages. The production of IL-6 and NO induced by the soluble factors from biofilm of mixed cultures was lower than that induced by the soluble factors from biofilm of single-species MSSA cultures, whereas the soluble factors from biofilm of C. albicans cultures induced only low levels of NO.
conclusionsSoluble factors from 36-h-old biofilm of C. albicans and MSSA cultures promoted cell death and inflammatory responses.
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