ArticleBiomedical optics express2017
Transient absorption imaging of hemes with 2-color, independently tunable visible-wavelength ultrafast source.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Statistical estimation theory detection limits for label-free imaging.Journal of biomedical optics · 2024Article
- Transient absorption spectroscopy and imaging of redox in muscle mitochondria.Biomedical optics express · 2022Article
- Adaptive noise canceling for transient absorption microscopy.Journal of biomedical optics · 2020Article
- Review
- Transient absorption microscopy: Technological innovations and applications in materials science and life science.The Journal of chemical physics · 2020Article
- Quantitative imaging of intraerythrocytic hemozoin by transient absorption microscopy.Journal of biomedical optics · 2019Article
- Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis.Science advances · 2019Article
- Label-Free Imaging of Heme Dynamics in Living Organisms by Transient Absorption Microscopy.Analytical chemistry · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pump probe microscopy is a time-resolved multiphoton imaging technique capable of generating contrast between non-fluorescent pigments based on differences in excited-state lifetimes. Here we describe a fiber-based ultrafast system designed for imaging heme proteins with an independently-tunable pulse pair in the visible-wavelength regime. Starting with a 1060 nm fiber amplifier (1.3 W at 63 MHz, 140 fs pulses), visible pulses were produced in the vicinity of 488 nm and 532 nm by doubling the output of a short photonic crystal fiber with a pair of periodically-poled lithium niobate crystals, providing 5-20 mW power in each beam. This was sufficient for acquiring transient absorption images from unstained cryosectioned tissue.
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