ArticleBiomedical optics express2017
Fluorescence quenching of free and bound NADH in HeLa cells determined by hyperspectral imaging and unmixing of cell autofluorescence.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Medical hyperspectral imaging: an updated review of technology advancements and biomedical applications.Journal of biomedical optics · 2026Pooled it
- Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2026Article
- MRS and Optical Imaging Studies of Therapeutic Response to Combination Therapy Targeting BRAF/MEK in Murine Melanomas.Academic radiology · 2026Article
- Investigating metabolic activity during oocyte and early embryo development through label-free metabolic imaging: a systematic approach for timelapse applications.Human reproduction (Oxford, England) · 2025Article
- Article
- Segmentation-guided photon pooling enables robust single cell analysis and fast fluorescence lifetime imaging microscopy.bioRxiv : the preprint server for biology · 2025Article
- Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling.Journal of biomedical optics · 2025Article
- Multispectral autofluorescence for label free classification of immune cell type and activation/polarization status.Scandinavian journal of immunology · 2025Article
- Novel application of metabolic imaging of early embryos using a light-sheet on-a-chip device: a proof-of-concept study.Human reproduction (Oxford, England) · 2025Article
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Syntheses and Applications of Coumarin-Derived Fluorescent Probes for Real-Time Monitoring of NAD(P)H Dynamics in Living Cells across Diverse Chemical Environments.ACS applied bio materials · 2024Article
- Applications of machine learning in time-domain fluorescence lifetime imaging: a review.Methods and applications in fluorescence · 2024Review
- Chemical and Cellular Formation of Reactive Oxygen Species from Secondary Organic Aerosols in Epithelial Lining Fluid.Research report (Health Effects Institute) · 2023Article
- Distinguishing Healthy and Carcinoma Cell Cultures Using Fluorescence Spectra Decomposition with a Genetic-Algorithm-Based Code.Biosensors · 2023Article
- Superoxide Release by Macrophages through NADPH Oxidase Activation Dominating Chemistry by Isoprene Secondary Organic Aerosols and Quinones to Cause Oxidative Damage on Membranes.Environmental science & technology · 2022Article
- Optical imaging detects metabolic signatures associated with oocyte quality†.Biology of reproduction · 2022Article
- Subcellular analysis of nuclear and cytoplasmic redox indices differentiates breast cancer cell subtypes better than nuclear-to-cytoplasmic area ratio.Journal of biomedical optics · 2022Article
- Multispectral autofluorescence characteristics of reproductive aging in old and young mouse oocytes.Biogerontology · 2022Article
- Article
- Non-invasive assessment of exfoliated kidney cells extracted from urine using multispectral autofluorescence features.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbonyl cyanide-p-trifluoro methoxyphenylhydrazone (FCCP) is a well-known mitochondrial uncoupling agent. We examined FCCP-induced fluorescence quenching of reduced nicotinamide adenine dinucleotide / nicotinamide adenine dinucleotide phosphate (NAD(P)H) in solution and in cultured HeLa cells in a wide range of FCCP concentrations from 50 to 1000µM. A non-invasive label-free method of hyperspectral imaging of cell autofluorescence combined with unsupervised unmixing was used to separately isolate the emissions of free and bound NAD(P)H from cell autofluorescence. Hyperspectral image analysis of FCCP-treated HeLa cells confirms that this agent selectively quenches fluorescence of free and bound NAD(P)H in a broad range of concentrations. This is confirmed by the measurements of average NAD/NADH and NADP/NADPH content in cells. FCCP quenching of free NAD(P)H in cells and in solution is found to be similar, but quenching of bound NAD(P)H in cells is attenuated compared to solution quenching possibly due to a contribution from the metabolic and/or antioxidant response in cells. Chemical quenching of NAD(P)H fluorescence by FCCP validates the results of unsupervised unmixing of cell autofluorescence.
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