ArticleScientific reports2017
A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 63 citations in OpenAlex.
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- Heterologous Expression of Recombinant Human Cytochrome P450 (CYP) inBiotech (Basel (Switzerland)) · 2023Review
- Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery.Microbial biotechnology · 2023Review
- Microbial Biocontainment Systems for Clinical, Agricultural, and Industrial Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Channelling carbon flux through the meta-cleavage route for improved poly(3-hydroxyalkanoate) production from benzoate and lignin-based aromatics in Pseudomonas putida H.Microbial biotechnology · 2021Article
- A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis.mSystems · 2021Article
- Biological Approaches in Polyhydroxyalkanoates Recovery.Current microbiology · 2021Review
- Fed-BatchFrontiers in bioengineering and biotechnology · 2021Article
- Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria.Nature communications · 2020Review
- Xylose-Inducible Promoter Tools forApplied and environmental microbiology · 2020Article
- Article
- Engineering the Osmotic State ofFrontiers in bioengineering and biotechnology · 2020Article
- Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion.Frontiers in bioengineering and biotechnology · 2020Review
- Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species.Microbial biotechnology · 2020Review
- Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers.Journal of industrial microbiology & biotechnology · 2019Article
- Dynamic control of endogenous metabolism with combinatorial logic circuits.Molecular systems biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. In this work, we developed a novel programmable lysis system based on the heterologous expression of lysozyme. The inducible lytic system was tested in two Gram-negative bacterial strains, namely Escherichia coli and Pseudomonas putida KT2440. Before induction, the lytic system did not significantly arrest essential physiological parameters in the recombinant E. coli (ECPi) and P. putida (JBOi) strain such as specific growth rate and biomass yield under standard growth conditions. A different scenario was observed in the recombinant JBOi strain when subjected to PHA-producing conditions, where biomass production was reduced by 25% but the mcl-PHA content was maintained at about 30% of the cell dry weight. Importantly, the genetic construct worked well under PHA-producing conditions (nitrogen-limiting phase), where more than 95% of the cell population presented membrane disruption 16 h post induction, with 75% of the total synthesized biopolymer recovered at the end of the fermentation period. In conclusion, this new lysis system circumvents traditional, costly mechanical and enzymatic cell-disrupting procedures.
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