Evidence map›Paper›PMID 28663331›Full record

ArticlePlant physiology2017

Multiplexed Gene Editing and Protein Overexpression Using a

Will B Cody, Herman B Scholthof, T Erik Mirkov

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Plant physiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 136 citations in OpenAlex.

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  8. CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Will B CodyDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77840.
Herman B ScholthofDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77840 erik.mirkov@ag.tamu.edu herscho@tamu.edu.ORCID http://orcid.org/0000-0002-3042-6534
T Erik MirkovDepartment of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77840 erik.mirkov@ag.tamu.edu herscho@tamu.edu.
Texas A&M University · USAdvanced Barber College & Hair Design · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Development of CRISPR/Cas9 transient gene editing screening tools in plant biology has been hindered by difficulty of delivering high quantities of biologically active single guide RNAs (sgRNAs). Furthermore, it has been largely accepted that in vivo generated sgRNAs need to be devoid of extraneous nucleotides, which has limited sgRNA expression by delivery vectors. Here, we increased cellular concentrations of sgRNA by transiently delivering sgRNAs using a

Indexed as

CRISPR-Cas SystemsTobacco Mosaic VirusGene EditingNicotiana

Identifiers

PMID28663331
PMCPMC5580747
OpenAlexW2728695862

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.