ArticleBMC research notes2017
A practical examination of RNA isolation methods for European pear (Pyrus communis).
Article in BMC research notes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Orchard and storage-dependent metabolic and transcriptional regulation of superficial scald in 'Packham's Triumph' pears.Frontiers in plant science · 2026Article
- Transcriptomics of long-term, low oxygen storage coupled with ethylene signaling interference suggests neofunctionalization of hypoxia response pathways in apple (Plant direct · 2024Article
- Towards identification of postharvest fruit quality transcriptomic markers in Malus domestica.PloS one · 2024Article
- FirstFrontiers in plant science · 2024Article
- Transcriptomic approach to uncover dynamic events in the development of mid-season sunburn in apple fruit.G3 (Bethesda, Md.) · 2023Article
- A phased, chromosome-scale genome of 'Honeycrisp' apple (GigaByte (Hong Kong, China) · 2022Article
- Transcriptomics of Differential Ripening in 'd'Anjou' Pear (Frontiers in plant science · 2021Article
- Selection of reference genes for RT-qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses.FEBS open bio · 2020Article
- Transcriptome sequencing of Himalayan Raspberry (3 Biotech · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveWith the goal of identifying fast, reliable, and broadly applicable RNA isolation methods in European pear fruit for downstream transcriptome analysis, we evaluated several commercially available kit-based RNA isolation methods, plus our modified version of a published cetyl trimethyl ammonium bromide (CTAB)-based method.
resultsWe confirmed previous work indicating chaotropic agent-based kits produced sufficient, high-quality RNA in freshly harvested, mature 'Bartlett' fruit. However, RNA isolation from 'd'Anjou' pear peel and especially cortical tissues of fruit stored for 11 months proved challenging to all but our modified CTAB-based method. Generally, more RNA was recovered from peel tissues than cortical tissues. Less toxic dithiothreitol was confirmed to be an acceptable reducing agent as its substitution for 2-mercaptoethanol often yielded high quality RNA. Finally, we present evidence that erroneous signals in the 5S region of Bioanalyzer RNA size plot histograms, that interfered with RNA integrity number calculation, were small RNA fragments that are reduced by simple cleanup procedures, not artifacts as previously reported.
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Registered trials
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