ArticlePloS one2017
Corneal epithelial cells exposed to shear stress show altered cytoskeleton and migratory behaviour.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
- Epithelial and Topographic Corneal Remodeling After Ptosis and Ectropion Surgery: An MS-39-Based Study.Diagnostics (Basel, Switzerland) · 2026Article
- Piezo1 Activation by Shear Stress Induces Corneal Epithelial Homeostasis Disruption.Investigative ophthalmology & visual science · 2026Article
- Influence of fluid shear stress on human limbal epithelial cells.Biochemistry and biophysics reports · 2026Article
- A theoretical and experimental model of flow characteristics in subretinal injections.PloS one · 2026Article
- Corneal Epithelial Tissue Engineering Strategy Based on Cell Viability Optimization: A Review and Prospects.Bioengineering (Basel, Switzerland) · 2025Review
- 3D-Printed Contact Lenses to Release Polyvinyl Alcohol as a Therapeutic Agent for the Treatment of Dry Eyes.Pharmaceutics · 2025Article
- The Contribution of the Limbus and Collagen Fibrils to Corneal Biomechanical Properties: Estimation of the Low-Strain In Vivo Elastic Modulus and Tissue Strain.Biomimetics (Basel, Switzerland) · 2024Article
- [Characterization of rabbit corneal biomechanical properties after corneal refractive surgery based on rapid loading-unloading uniaxial tensile test].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2024Article
- A Microfluidic Platform for the Time-Resolved Interrogation of Polarized Retinal Pigment Epithelial Cells.Translational vision science & technology · 2023Article
- Response of cells and tissues to shear stress.Journal of cell science · 2023Review
- Metabolic reprogramming in response to cell mechanics.Biology of the cell · 2023Review
- Transmembrane Mucin Response in Conjunctival Epithelial Cells Exposed to Wall Shear Stresses.International journal of molecular sciences · 2023Article
- Article
- Marangoni effect and cell spreading.European biophysics journal : EBJ · 2022Review
- A More Biomimetic Cell Migration Assay with High Reliability and Its Applications.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Cell senescence alters responses of porcine trabecular meshwork cells to shear stress.Frontiers in cell and developmental biology · 2022Article
- Unraveling the mechanobiology of cornea: From bench side to the clinic.Frontiers in bioengineering and biotechnology · 2022Review
- Functional morphology of the cornea of the Little Penguin Eudyptula minor (Aves).Journal of anatomy · 2021Article
- Bioscaffolds embedded with regulatory modules for cell growth and tissue formation: A review.Bioactive materials · 2021Review
- The biomechanical basis of biased epithelial tube elongation in lung and kidney development.Development (Cambridge, England) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells that form the corneal epithelium, the outermost layer of the cornea, are exposed to shear stress through blinking during waking hours. In this in vitro study, the effect of fluid shear stress on human corneal epithelial cells (HCECs) was investigated. Following exposure to shear stresses of 4 and 8 dyn/cm2, HCECs showed cytoskeletal rearrangement with more prominent, organized and elongated filamentous actin. Cytoskeletal changes were time-dependent, and were most significant after 24 hours of shear stress. Higher rates of migration and proliferation, as evaluated by a scratch assay, were also observed following 24 hours of low shear stress exposure (4 dyn/cm2). This result contrasted the poor migration observed in samples scratched before shear exposure, indicating that shear-induced cytoskeletal changes played a key role in improved wound healing and must therefore precede any damage to the cell layer. HCEC cytoskeletal changes were accompanied by an upregulation in integrin β1 and downregulation of ICAM-1. These results demonstrate that HCECs respond favourably to flow-induced shear stress, impacting their proliferation and migration properties as well as phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.