ArticlePloS one2017
A new approach for the assessment of the toxicity of polyphenol-rich compounds with the use of high content screening analysis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
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- p-Sulfonatocalixarene-Based Complexation Mitigates Hepatorenal Toxicity of Anticancer Alkaloids without Loss of Efficacy: A Strategy for Selective Detoxification.The AAPS journal · 2026Article
- Integrated green extraction of phenolic compounds and fermentable sugars from grapefruit peel using ultrasound and enzymatic hydrolysis.Journal of the science of food and agriculture · 2026Article
- Preclinical Risk Assessment of Plant Lectins with Pharmacological Applications: A Narrative Review.Molecules (Basel, Switzerland) · 2025Review
- Inhibitory Effects of 3-(4-Hydroxy-3-methoxyphenyl) Propionic Acid on Amyloid β-Peptide Aggregation In Vitro.Biomedicines · 2025Article
- Polyphenols: From Classification to Therapeutic Potential and Bioavailability.Foods (Basel, Switzerland) · 2024Review
- Phenolic Fractions from Walnut Milk Residue: Antioxidant Activity and Cytotoxic Potential.Plants (Basel, Switzerland) · 2024Article
- Antibacterial, Antifungal, Antiviral Activity, and Mechanisms of Action of Plant Polyphenols.Microorganisms · 2024Review
- Sub-chronic and acute toxicity of aqueous extractsToxicology reports · 2024Article
- Preparation of Functional Food with Enhanced Antioxidant Properties by AddingFoods (Basel, Switzerland) · 2024Article
- Regulatory mechanism and therapeutic potentials of naringin against inflammatory disorders.Heliyon · 2024Review
- Rowanberry-A Source of Bioactive Compounds and Their Biopharmaceutical Properties.Plants (Basel, Switzerland) · 2023Review
- Influence of Coffee Variety and Processing on the Properties of Parchments as Functional Bioadditives for Biobased Poly(Polymers · 2023Article
- Towards chemical characterization and possible applications of juçara fruit: an approach to removeJournal of food science and technology · 2023Review
- Anxiolytic and Antioxidant Effect of Phytoecdysteroids and Polyphenols fromMolecules (Basel, Switzerland) · 2022Article
- Composition of Nonextractable Polyphenols from Sweet Cherry Pomace Determined by DART-Orbitrap-HRMS and TheirJournal of agricultural and food chemistry · 2022Article
- The Involvement of Natural Polyphenols in the Chemoprevention of Cervical Cancer.International journal of molecular sciences · 2021Review
- Review
- A phenolic-rich extract from Ugni molinae berries reduces abnormal protein aggregation in a cellular model of Huntington's disease.PloS one · 2021Article
- Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds.Arhiv za higijenu rada i toksikologiju · 2020Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The toxicity of in vitro tested compounds is usually evaluated based on AC50 values calculated from dose-response curves. However, there is a large group of compounds for which a standard four-parametric sigmoid curve fitting may be inappropriate for estimating AC50. In the present study, 22 polyphenol-rich compounds were prioritized from the least to the most toxic based on the total area under and over the dose-response curves (AUOC) in relation to baselines. The studied compounds were ranked across three key cell indicators (mitochondrial membrane potential, cell membrane integrity and nuclear size) in a panel of five cell lines (HepG2, Caco-2, A549, HMEC-1, and 3T3), using a high-content screening (HCS) assay. Regarding AUOC score values, naringin (negative control) was the least toxic phenolic compound. Aronox, spent hop extract and kale leaf extract had very low cytotoxicity with regard to mitochondrial membrane potential and cell membrane integrity, as well as nuclear morphology (nuclear area). Kaempferol (positive control) exerted strong cytotoxic effects on the mitochondrial and nuclear compartments. Extracts from buckthorn bark, walnut husk and hollyhock flower were highly cytotoxic with regard to the mitochondrion and cell membrane, but not the nucleus. We propose an alternative algorithm for the screening of a large number of agents and for identifying those with adverse cellular effects at an early stage of drug discovery, using high content screening analysis. This approach should be recommended for series of compounds producing a non-sigmoidal cell response, and for agents with unknown toxicity or mechanisms of action.
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