Evidence map›Paper›PMID 28660572›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Sample Preparation and Analysis for Imaging Mass Spectrometry.

Genea Edwards, Annia Mesa, Robert I Vazquez-Padron, Jane-Marie Kowalski, Sanjoy K Bhattacharya

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Genea EdwardsBascom Palmer Eye Institute, University of Miami, Miami, FL, USA.
Annia MesaDepartment of Surgery and Vascular Biology Institute, University of Miami, Miami, FL, 33136, USA.
Robert I Vazquez-PadronDepartment of Surgery and Vascular Biology Institute, University of Miami, Miami, FL, 33136, USA.
Jane-Marie KowalskiBruker Daltonics Inc., Billerica, MA, 01821, USA.
Sanjoy K BhattacharyaDepartment of Biochemistry and Molecular Biology, University of Miami, Miami, FL, 33136, USA. sbhattacharya@med.miami.edu.
University of Miami · USBruker (United States) · US

Funding

University of Miami Computational Ocular Genomics Training ProgramT32EY023194 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI SCOTT, WILLIAM K · 2013 to 2017
$661k
NEI NIH HHS T32 EY023194
6 · The paper itself

Abstract

Imaging mass spectrometry (IMS) is a novel quantitative technique used to investigative diverse biomolecules in tissue sections. Specifically, IMS uses analytical separation of mass spectrometry to determine the spatial distribution of certain lipids and/or proteins located directly on biological sections from a single tissue sample. Typically, IMS is combined with histological analysis to reveal additional distribution details of characterized biomolecules including cell type and/or subcellular localization. In this chapter, we describe the use of Matrix-Assisted Laser Desorption/Ionization (MALDI) Time-Of-Flight/Time-Of-Flight (TOF/TOF) to analyze various cholesterol and phosphatidylcholine species in atherosclerotic plaque of murine heart aortic valves. In particular, we detail animals used, tissue collection, preparation, matrix application, spectra acquisition for generating a color-coded image based on IMS spectral characteristics.

Indexed as

AnimalsApolipoproteins EBiomarkersFemaleMass SpectrometryMetabolomicsMiceMice, KnockoutSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationApolipoproteins EBiomarkersBiomarkerImaging mass spectrometry (IMS)Matrix-assisted laser desorption ionization (MALDI)MetaboliteTime-of-flight (TOF)

Identifiers

PMID28660572
PMCPMC7547848
OpenAlexW2729909795

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.