Evidence map›Paper›PMID 28659899›Full record

ArticleFrontiers in microbiology2017

A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies.

Marco Fabiani, Dolores Limongi, Anna Teresa Palamara, Giovanna De Chiara, Maria Elena Marcocci

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus.Journal of visualized experiments : JoVE · 2021
    Article
  5. Article
  6. Article
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  11. The Amphibian Antimicrobial Peptide Temporin B InhibitsAntimicrobial agents and chemotherapy · 2018
    Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Marco FabianiDepartment of Public Health and Infectious Diseases, Sapienza University of RomeRome, Italy.
Dolores LimongiSan Raffaele Pisana, Istituto di Ricovero e Cura a Carattere Scientifico, Telematic UniversityRome, Italy.
Anna Teresa PalamaraSan Raffaele Pisana, Istituto di Ricovero e Cura a Carattere Scientifico, Telematic UniversityRome, Italy.
Giovanna De ChiaraInstitute of Translational Pharmacology, National Research CouncilRome, Italy.
Maria Elena MarcocciDepartment of Public Health and Infectious Diseases, Sapienza University of RomeRome, Italy.
Sapienza University of Rome · ITIstituti di Ricovero e Cura a Carattere Scientifico · ITIstituto di Farmacologia Traslazionale · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Among several strategies used for Herpes simplex virus (HSV) detection in biological specimens, standard plaque assay (SPA) remains the most reliable method to evaluate virus infectivity and quantify viral replication. However, it is a manual procedure, thereby affected by operator subjectivity, and it may be particularly laborious for multiple sample analysis. Here we describe an innovative method to perform the titration of HSV type 1 (HSV-1) in different samples, using the "In-Cell Western

Indexed as

antiviralsherpes simplex virusHSV-1immunostainingnear-infrared fluorescenceplaque assayvirus titration

Identifiers

PMID28659899
PMCPMC5469900
OpenAlexW2626936758

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.