ArticleScientific reports2017
Site-specific gene expression analysis using an automated tissue micro-dissection punching system.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Divergent and subnucleus-specific gene expression responses to chronic stress hormone exposure in the amygdala.Frontiers in molecular neuroscience · 2025Article
- Spatial transcriptomic profiling of isolated microregions in tissue sections utilizing laser-induced forward transfer.PloS one · 2024Article
- Identification of target cells of human papillomavirus 18 using squamocolumnar junction organoids.Cancer science · 2024Article
- Spatial transcriptomics in development and disease.Molecular biomedicine · 2023Review
- Cells with stem-like properties are associated with the development of HPV18-positive cervical cancer.Cancer science · 2023Article
- Reproducible and sensitive micro-tissue RNA sequencing from formalin-fixed paraffin-embedded tissues for spatial gene expression analysis.Scientific reports · 2022Article
- Identification of two cancer stem cell-like populations in triple-negative breast cancer xenografts.Disease models & mechanisms · 2022Article
- An efficient procedure for the recovery of DNA from formalin-fixed paraffin-embedded tissue sections.Biology methods & protocols · 2022Article
- Integrated spatial analysis of gene mutation and gene expression for understanding tumor diversity in formalin-fixed paraffin-embedded lung adenocarcinoma.Frontiers in oncology · 2022Article
- Cortical transcriptome analysis after spinal cord injury reveals the regenerative mechanism of central nervous system in CRMP2 knock-in mice.Neural regeneration research · 2021Article
- Spatially mapped single-cell chromatin accessibility.Nature communications · 2021Article
- Distinctive Regulation of Emotional Behaviors and Fear-Related Gene Expression Responses in Two Extended Amygdala Subnuclei With Similar Molecular Profiles.Frontiers in molecular neuroscience · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Site-specific gene expression analyses are important for understanding tissue functions. Despite rapid developments in DNA-related technologies, the site-specific analysis of whole genome expression for a tissue remains challenging. Thus, a new tool is required for capturing multiple tissue micro-dissections or single cells while retaining the positional information. Here, we describe the development of such a system, which can pick up micro-dissections by punching a tissue repeatedly in a very short period, e.g., 5 s/sampling cycle. A photo of the punched tissue provides information on the dissected positions, allowing site-specific gene expression analysis. We demonstrate the site-specific analysis of a frozen tissue slice of mouse brain by analyzing many micro-dissections produced from the tissue at a 300-μm pitch. The site-specific analysis provided new insights into the gene expression profiles in a tissue and on tissue functions. The analysis of site-specific whole genome expression may therefore, open new avenues in life science.
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