ArticleScientific reports2017
A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Metabolic engineering ofMetabolic engineering communications · 2026Article
- Cell-Free-Based Thermophilic Biocatalyst for the Synthesis of Amino Acids from One-Carbon Feedstocks.ACS synthetic biology · 2025Article
- Improvement of Soluble Expression, Stability, and Activity of Acetaldehyde Lyase by Elastin-like Polypeptides Fusion for Acetoin Production from Acetaldehyde.Biomolecules · 2025Article
- Process optimization of co-fermentation natto withJournal of food science and technology · 2025Article
- Effects of Different Drying Processes on Bioactive Components, Volatile Compounds, and In Vitro Inhibition of Starch Digestion in Mulberry Leaf Extracts.Foods (Basel, Switzerland) · 2025Article
- Metabolic engineering ofApplied and environmental microbiology · 2024Article
- Genetic engineering of a thermophilic acetogen,Frontiers in bioengineering and biotechnology · 2024Article
- Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones.Scientific reports · 2020Article
- A Genome-Scale Metabolic Model of 2,3-Butanediol Production by Thermophilic BacteriaMicroorganisms · 2020Article
- Increasing Yield of 2,3,5,6-Tetramethylpyrazine in Baijiu ThroughFrontiers in microbiology · 2020Article
- Structure-Based Design of Acetolactate Synthase FromFrontiers in microbiology · 2020Article
- Extending Designed Linear Biocatalytic Cascades for Organic Synthesis.ChemCatChem · 2019Review
- Simultaneous biosynthesis of (Metabolic engineering communications · 2018Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetoin (3-hydroxy-2-butanone) is an important bio-based platform chemical with wide applications. In vitro enzyme catalysed synthesis exhibits great feasibility in the production of chemicals with high purity. In the present work, a synthetic pathway involving a two-step continuous reaction was constructed in vitro for acetoin production from pyruvate at improved temperature. Thermostable candidates, acetolactate synthase (coAHASL1 and coAHASL2 from Caldicellulosiruptor owensensis OL) and α-acetolactate decarboxylase (bsALDC from Bacillus subtilis IPE5-4) were cloned, heterologously expressed, and characterized. All the enzymes showed maximum activities at 65-70 °C and pH of 6.5. Enzyme kinetics analysis showed that coAHASL1 had a higher activity but lower affinity against pyruvate than that of coAHASL2. In addition, the activities of coAHASL1 and bsALDC were promoted by Mn
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