ArticleThe Journal of biological chemistry2017
The long non-coding RNA GAS5 regulates transforming growth factor β (TGF-β)-induced smooth muscle cell differentiation via RNA Smad-binding elements.
Article in The Journal of biological chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- Long noncoding RNA GAS5 disrupts intestinal epithelial barrier function by increasing small vault RNA levels.JCI insight · 2026Article
- Serum lncRNAs NEAT1, PVT1 and H19 as novel biomarkers for sarcopenia diagnosis and treatment response.Non-coding RNA research · 2025Article
- LncRNA TP53TG1 promotes the growth and osteo/dentinogenic differentiation of dental pulp stem cells by activating the Smad3 and JNK1/2 pathway.In vitro cellular & developmental biology. Animal · 2025Article
- Epigenetic Regulation by lncRNA GAS5/miRNA/mRNA Network in Human Diseases.International journal of molecular sciences · 2025Review
- Roles of non-coding RNAs in intercellular crosstalk in cardiovascular diseases.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2023Review
- TGF-β receptor I inhibitor may restrict the induction of EMT in inflamed intestinal epithelial cells.Experimental biology and medicine (Maywood, N.J.) · 2023Article
- LINC00152 induced by TGF-β promotes metastasis via HuR in lung adenocarcinoma.Cell death & disease · 2022Article
- Circular RNAs in cardiovascular diseases.Pharmacology & therapeutics · 2022Review
- Long Noncoding RNAMolecular and cellular biology · 2022Article
- CircSOD2: A Novel Regulator for Smooth Muscle Proliferation and Neointima Formation.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- Review
- Janus Kinase 3 Deficiency Promotes Vascular Reendothelialization-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- A novel lincRNA identified in buffalo oocytes with protein binding characteristics could hold the key for oocyte competence.Molecular biology reports · 2021Article
- Functional Network of the Long Non-coding RNA Growth Arrest-Specific Transcript 5 and Its Interacting Proteins in Senescence.Frontiers in genetics · 2021Article
- GATA6-AS1 Regulates GATA6 Expression to Modulate Human Endoderm Differentiation.Stem cell reports · 2020Article
- LncRNA GAS5 attenuates fibroblast activation through inhibiting Smad3 signaling.American journal of physiology. Cell physiology · 2020Article
- Long noncoding RNAs in key cellular processes involved in aortic aneurysms.Atherosclerosis · 2020Review
- Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma.Cell death & disease · 2019Article
- The TGFB2-AS1 lncRNA Regulates TGF-β Signaling by Modulating Corepressor Activity.Cell reports · 2019Article
- LncRNA GAS5 regulates vascular smooth muscle cell cycle arrest and apoptosis via p53 pathway.Biochimica et biophysica acta. Molecular basis of disease · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Smooth muscle cell (SMC) differentiation is essential for vascular development, and TGF-β signaling plays a critical role in this process. Although long non-coding RNAs (lncRNAs) regulate various cellular events, their functions in SMC differentiation remain largely unknown. Here, we demonstrate that the lncRNA growth arrest-specific 5 (GAS5) suppresses TGF-β/Smad3 signaling in smooth muscle cell differentiation of mesenchymal progenitor cells. We found that forced expression of GAS5 blocked, but knockdown of GAS5 increased, the expression of SMC contractile proteins. Mechanistically, GAS5 competitively bound Smad3 protein via multiple RNA Smad-binding elements (rSBEs), which prevented Smad3 from binding to SBE DNA in TGF-β-responsive SMC gene promoters, resulting in suppression of SMC marker gene transcription and, consequently, in inhibition of TGF-β/Smad3-mediated SMC differentiation. Importantly, other lncRNAs or artificially synthesized RNA molecules that contained rSBEs also effectively inhibited TGF-β/Smad3 signaling, suggesting that lncRNA-rSBE may be a general mechanism used by cells to fine-tune Smad3 activity in both basal and TGF-β-stimulated states. Taken together, our results have uncovered an lncRNA-based mechanism that modulates TGF-β/Smad3 signaling during SMC differentiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.