ArticleCell reports2017
Functional Dissection of the Pol V Largest Subunit CTD in RNA-Directed DNA Methylation.
Article in Cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- La protein binding to telomerase RNA supports an evolutionary relationship between plant and ciliate telomerase pathways.Nucleic acids research · 2026Article
- A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis.Nature communications · 2024Article
- DNA-dependent RNA polymerases in plants.The Plant cell · 2023Article
- The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Genetic and environmental modulation of transposition shapes the evolutionary potential of Arabidopsis thaliana.Genome biology · 2021Article
- Article
- Multifaceted roles of RNA polymerase IV in plant growth and development.Journal of experimental botany · 2020Article
- Viral silencing suppressors and cellular proteins partner with plant RRP6-like exoribonucleases.Virus genes · 2020Article
- Common alleles of CMT2 and NRPE1 are major determinants of CHH methylation variation in Arabidopsis thaliana.PLoS genetics · 2019Article
- The Pol IV largest subunit CTD quantitatively affects siRNA levels guiding RNA-directed DNA methylation.Nucleic acids research · 2019Article
- Evidence for a Unique DNA-Dependent RNA Polymerase in Cereal Crops.Molecular biology and evolution · 2018Article
- RNA polymerases IV and V influence the 3' boundaries of Polymerase II transcription units in Arabidopsis.RNA biology · 2018Article
- Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Non-coding RNA polymerases that silence transposable elements and reprogram gene expression in plants.TranscriptionReview
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Authors and funding
6 authors.
Funding
Abstract
Plant multisubunit RNA polymerase V (Pol V) transcription recruits Argonaute-small interfering RNA (siRNA) complexes that specify sites of RNA-directed DNA methylation (RdDM) for gene silencing. Pol V's largest subunit, NRPE1, evolved from the largest subunit of Pol II but has a distinctive C-terminal domain (CTD). We show that the Pol V CTD is dispensable for catalytic activity in vitro yet essential in vivo. One CTD subdomain (DeCL) is required for Pol V function at virtually all loci. Other CTD subdomains have locus-specific effects. In a yeast two-hybrid screen, the 3'→ 5' exoribonuclease RRP6L1 was identified as an interactor with the DeCL and glutamine-serine (QS)-rich subdomains located downstream of an Argonaute-binding subdomain. Experimental evidence indicates that RRP6L1 trims the 3' ends of Pol V transcripts sliced by Argonaute 4 (AGO4), suggesting a model whereby the CTD enables the spatial and temporal coordination of AGO4 and RRP6L1 RNA processing activities.
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