ArticlePloS one2017
Red rot resistant transgenic sugarcane developed through expression of β-1,3-glucanase gene.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Emerging Fungal Pathogens Warfare, Fungi-Like Organisms, and the World's Major Crops: Lessons from the Past and Solutions for the Future.Current microbiology · 2025Review
- Enhancing quality and climate resilient traits in vegetatively propagated polyploids: transgenic and genome editing advancements, challenges and future directions.Frontiers in genetics · 2025Review
- Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Expression of Trichoderma spp. endochitinase gene improves red rot disease resistance in transgenic sugarcane.PloS one · 2024Article
- Factors affecting the production of sugarcane yield and sucrose accumulation: suggested potential biological solutions.Frontiers in plant science · 2024Review
- Genetic engineering: an efficient approach to mitigating biotic and abiotic stresses in sugarcane cultivation.Plant signaling & behavior · 2022Article
- Conversion of sheath blight susceptible indica and japonica rice cultivars into moderately resistant through expression of antifungal β-1,3-glucanase transgene from Trichoderma spp.Transgenic research · 2022Article
- Review
- A short review on sugarcane: its domestication, molecular manipulations and future perspectives.Genetic resources and crop evolution · 2022Review
- Basic β-1,3-Glucanase fromPlants (Basel, Switzerland) · 2021Article
- Over-Expression of Endogenous SUGARWIN Genes Exalted Tolerance againstPlants (Basel, Switzerland) · 2021Article
- Genetic Transformation of Sugarcane, Current Status and Future Prospects.Frontiers in plant science · 2021Review
- Applying Molecular Phenotyping Tools to Explore Sugarcane Carbon Potential.Frontiers in plant science · 2021Review
- Foot rot tolerant transgenic rough lemon rootstock developed through expression of β-1,3-glucanase from Trichoderma spp.Plant biotechnology journal · 2019Article
- Antifungal activity of chitinase II against Colletotrichum falcatum Went. causing red rot disease in transgenic sugarcane.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sugarcane (Saccharum spp.) is a commercially important crop, vulnerable to fungal disease red rot caused by Colletotrichum falcatum Went. The pathogen attacks sucrose accumulating parenchyma cells of cane stalk leading to severe losses in cane yield and sugar recovery. We report development of red rot resistant transgenic sugarcane through expression of β-1,3-glucanase gene from Trichoderma spp. The transgene integration and its expression were confirmed by quantitative reverse transcription-PCR in first clonal generation raised from T0 plants revealing up to 4.4-fold higher expression, in comparison to non-transgenic sugarcane. Bioassay of transgenic plants with two virulent C. falcatum pathotypes, Cf 08 and Cf 09 causing red rot disease demonstrated that some plants were resistant to Cf 08 and moderately resistant to Cf 09. The electron micrographs of sucrose storing stalk parenchyma cells from these plants displayed characteristic sucrose-filled cells inhibiting Cf 08 hyphae and lysis of Cf 09 hyphae; in contrast, the cells of susceptible plants were sucrose depleted and prone to both the pathotypes. The transgene expression was up-regulated (up to 2.0-fold in leaves and 5.0-fold in roots) after infection, as compared to before infection in resistant plants. The transgene was successfully transmitted to second clonal generation raised from resistant transgenic plants. β-1,3-glucanase protein structural model revealed that active sites Glutamate 628 and Aspartate 569 of the catalytic domain acted as proton donor and nucleophile having role in cleaving β-1,3-glycosidic bonds and pathogen hyphal lysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.