ArticlePLoS genetics2017
One ligand, two regulators and three binding sites: How KDPG controls primary carbon metabolism in Pseudomonas.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- The Role of Glyceraldehyde-3-Phosphate Dehydrogenase in 2-Ketogluconic Acid Industrial Production StrainFoods (Basel, Switzerland) · 2025Article
- Functional genomics of chitin degradation by Vibrio parahaemolyticus reveals finely integrated metabolic contributions to support environmental fitness.PLoS genetics · 2025Article
- A chromosomal mutation is superior to a plasmid-encoded mutation for plasmid fitness cost compensation.PLoS biology · 2024Article
- The Functional Characterization of the 6-Phosphogluconate Dehydratase Operon in 2-Ketogluconic Acid Industrial Producing StrainFoods (Basel, Switzerland) · 2024Article
- Molecular mechanism of the one-component regulator RccR on bacterial metabolism and virulence.Nucleic acids research · 2024Article
- The genotype of barley cultivars influences multiple aspects of their associated microbiota via differential root exudate secretion.PLoS biology · 2024Article
- A KDPG sensor RccR governs Pseudomonas aeruginosa carbon metabolism and aminoglycoside antibiotic tolerance.Nucleic acids research · 2024Article
- Study of peripheral domains in structure-function of isocitrate lyase (ICL) from Pseudomonas aeruginosa.World journal of microbiology & biotechnology · 2023Article
- Plasmids manipulate bacterial behaviour through translational regulatory crosstalk.PLoS biology · 2023Article
- Metabolic Mechanism and Physiological Role of Glycerol 3-Phosphate in Pseudomonas aeruginosa PAO1.mBio · 2022Article
- Engineering the glyoxylate cycle for chemical bioproduction.Frontiers in bioengineering and biotechnology · 2022Review
- Entner-Doudoroff pathway inFrontiers in microbiology · 2022Article
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- Inducible and tunable gene expression systems for Pseudomonas putida KT2440.Scientific reports · 2021Article
- Genome-wide association study of signature genetic alterations among pseudomonas aeruginosa cystic fibrosis isolates.PLoS pathogens · 2021Article
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- Expression of the ace operon in Escherichia coli is triggered in response to growth rate-dependent flux-signal of ATP.FEMS microbiology letters · 2021Article
- Pectobacterium atrosepticum KDPG aldolase, Eda, participates in the Entner-Doudoroff pathway and independently inhibits expression of virulence determinants.Molecular plant pathology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effective regulation of primary carbon metabolism is critically important for bacteria to successfully adapt to different environments. We have identified an uncharacterised transcriptional regulator; RccR, that controls this process in response to carbon source availability. Disruption of rccR in the plant-associated microbe Pseudomonas fluorescens inhibits growth in defined media, and compromises its ability to colonise the wheat rhizosphere. Structurally, RccR is almost identical to the Entner-Doudoroff (ED) pathway regulator HexR, and both proteins are controlled by the same ED-intermediate; 2-keto-3-deoxy-6-phosphogluconate (KDPG). Despite these similarities, HexR and RccR control entirely different aspects of primary metabolism, with RccR regulating pyruvate metabolism (aceEF), the glyoxylate shunt (aceA, glcB, pntAA) and gluconeogenesis (pckA, gap). RccR displays complex and unusual regulatory behaviour; switching repression between the pyruvate metabolism and glyoxylate shunt/gluconeogenesis loci depending on the available carbon source. This regulatory complexity is enabled by two distinct pseudo-palindromic binding sites, differing only in the length of their linker regions, with KDPG binding increasing affinity for the 28 bp aceA binding site but decreasing affinity for the 15 bp aceE site. Thus, RccR is able to simultaneously suppress and activate gene expression in response to carbon source availability. Together, the RccR and HexR regulators enable the rapid coordination of multiple aspects of primary carbon metabolism, in response to levels of a single key intermediate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.