Evidence map›Paper›PMID 28656079›Full record

ArticleIranian journal of basic medical sciences2017

Construction, expression, purification and characterization of secretin domain of PilQ and triple PilA-related disulfide loop peptides fusion protein from

Sobhan Faezi, Ahmad Reza Bahrmand, Seyed Davar Siadat, Iraj Nikokar, Soroush Sardari, Mehdi Mahdavi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Iranian journal of basic medical sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. UnderstandingCells · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Sobhan FaeziDepartments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Ahmad Reza BahrmandDepartments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Seyed Davar SiadatDepartments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Iraj NikokarDepartment of Biotechnology, Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.
Soroush SardariBiotechnology Research Center, Drug Design and Bioinformatics Group, Pasteur Institute of Iran, Tehran, Iran.
Mehdi MahdaviDepartments of Immunology, Pasteur Institute of Iran, Tehran, Iran.
Pasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesInfection with MATERIALS AND

methodsWe surveyed the expression of the PilQ

resultsThe fusion protein was purified in high yield by osmotic shock method using HisTrap affinity column. The protein was confirmed by immunoblot analysis. The checkerboard titration showed that the optimal dilution of the antibody to react with antigen is 1:128. Results of opsonophagocytosis assay revealed that the antibodies elevated to the fusion protein promoted phagocytosis of the PAO1 and 6266E strains, so that the twitching immobilization test confirmed these results.

conclusionDue to excellent killing activity mediated by opsonic antibodies and efficient immobilization of the strains, it seems that PilQ

Indexed as

Fusion proteinpET26bPilAPilQPseudomonas aeruginosaType IV pili

Identifiers

PMID28656079
PMCPMC5478772
OpenAlexW2624942094

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.