Evidence map›Paper›PMID 28655663›Full record

ArticleMitochondrion2017

Resistive flow sensing of vital mitochondria with nanoelectrodes.

Katayoun Zand, Ted D A Pham, Jinfeng Li, Weiwei Zhou, Douglas C Wallace, Peter J Burke

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mitochondrion, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. How defective mitochondrial electrical activity leads to inherited blindness.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  3. Micro- and Nano-Devices for Studying Subcellular Biology.Small (Weinheim an der Bergstrasse, Germany) · 2021
    Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Katayoun ZandIntegrated Nanosystems Research Facility, Electrical Engineering and Computer Science, University of California, Irvine, Irvine, CA 92697, United States.
Ted D A PhamDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, United States.
Jinfeng LiIntegrated Nanosystems Research Facility, Electrical Engineering and Computer Science, University of California, Irvine, Irvine, CA 92697, United States.
Weiwei ZhouIntegrated Nanosystems Research Facility, Electrical Engineering and Computer Science, University of California, Irvine, Irvine, CA 92697, United States.
Douglas C WallaceCenter for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia and Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States.
Peter J BurkeIntegrated Nanosystems Research Facility, Electrical Engineering and Computer Science, University of California, Irvine, Irvine, CA 92697, United States. Electronic address: pburke@uci.edu.

Funding

MITOCHONDRIAL INBORN ERRORS OF METABOLISMR01NS021328 · NINDS · UNIVERSITY OF CALIFORNIA-IRVINE · PI WALLACE, DOUGLAS C · 1985 to 2017
$6.5M
High-throughput on-chip platform for interrogation of mitochondrial membrane potential and apoptosisR33CA182384 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI BURKE, PETER J. · 2015 to 2017
$1.2M
Nanoelectrode arrays for study of the molecular mechanisms, triggers, and inhibitR21CA143351 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI BURKE, PETER J. · 2010 to 2012
$631k
NCI NIH HHS R21 CA143351NCI NIH HHS R33 CA182384NINDS NIH HHS R01 NS021328
6 · The paper itself

Abstract

We report label-free detection of single mitochondria with high sensitivity using nanoelectrodes. Measurements of the conductance of carbon nanotube transistors show discrete changes of conductance as individual mitochondria flow over the nanoelectrodes in a microfluidic channel. Altering the bioenergetic state of the mitochondria by adding metabolites to the flow buffer induces changes in the mitochondrial membrane potential detected by the nanoelectrodes. During the time when mitochondria are transiently passing over the nanoelectrodes, this (nano) technology is sensitive to fluctuations of the mitochondrial membrane potential with a resolution of 10mV with temporal resolution of order milliseconds. Fluorescence based assays (in ideal, photon shot noise limited setups) are shown to be an order of magnitude less sensitive than this nano-electronic measurement technology. This opens a new window into the dynamics of an organelle critical to cellular function and fate.

Indexed as

ElectrodesEnergy MetabolismMembrane Potential, MitochondrialHeLa CellsHumansMicrofluidicsMitochondriaNanotechnologyNanotubesBioenergeticsCarbon nanotubeLabel-freeMitochondriaResistive sensing

Identifiers

PMID28655663
PMCPMC6377799

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.