Evidence map›Paper›PMID 28655353›Full record

ArticleBMC microbiology2017

XC_0531 encodes a c-type cytochrome biogenesis protein and is required for pathogenesis in Xanthomonas campestris pv. campestris.

Lei Chen, Mingpeng Wang, Li Huang, Zhaojie Zhang, Fanghua Liu, Guangtao Lu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. mBio · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Lei ChenState Key Laboratory for Conservation and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, The Key Laboratory ofMinistry of Education for Microbial and Plant Genetic Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, China.
Mingpeng WangKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Yantai, China.
Li HuangState Key Laboratory for Conservation and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, The Key Laboratory ofMinistry of Education for Microbial and Plant Genetic Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, China.
Zhaojie ZhangDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY, USA.
Fanghua LiuKey Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Yantai, China.
Guangtao LuState Key Laboratory for Conservation and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, The Key Laboratory ofMinistry of Education for Microbial and Plant Genetic Engineering, Guangxi University, 100 Daxue Road, Nanning, Guangxi, 530004, China. lugt@gxu.edu.cn.
State Key Laboratory For Conservation and Utilization of Subtropical Agro-BioresourcesYantai Institute of Coastal Zone Research · CNGuangxi University · CNUniversity of Wyoming · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe phytopathogenic Xanthomonas campestris pv.campestris is a gram-negative bacterium and the causal agent of black-rot disease of cruciferous crops. Many gram-negative bacteria possess a family of proteins, called Dsbs, which are involved in disulfide bond formation in certain periplasmic proteins. In our preliminary screening of the virulence to the plants we identified that gene XC_0531 which annotated gene dsbD of Xanthomonas campestris pv. campestris (Xcc) is related to the virulence to the host plants.

resultsHere, we found XC_0531 encoded a DsbD like protein. Its deletion is sensitive to DTT and copper, decreased accumulation of free thiols in periplasm. Its deletion also affected heme synthesis, position of Soret band and the production of peak c550. This suggests that XC_0531 is related to c-type cytochromes biogenesis. XC_0531 mutation decreased the utilization of different carbon sources (such as galactose, xylose, maltose, saccharose and glucose), reduced extracellular polysaccharide (EPS) production, decreased extracellular enzyme activities (protease, cellulose and amylase), slowed down growth rate of Xcc and weakened virulence to the plants. These results suggest that these phenotypes caused by XC_0531 mutation is possibly due to deficient biosynthesis of c-type cytochromes in respiration chain and the formation of disulfide bonds. Our work confirmed the function of XC_0531 and provide theory basis for scientists working on molecular mechanisms of cytochrome c biogenesis, pathogenesis of Xcc, development of EPS commercial values and protecting plant from black rot.

conclusionWe confirmed the function of gene XC_0531, which encodes a DsbD like protein, a protein correlated with c-type cytochrome biogenesis. This gene is related to the virulence to plants by affecting funtion of cytochromes c and probably disulfide bonds modification of proteins in type II secretion system (T2SS).

Indexed as

Bacterial ProteinsCarbonCytochromes cDisulfidesGene Expression Regulation, BacterialGene Expression Regulation, EnzymologicMutationOxidoreductasesRaphanusVirulenceXanthomonas campestrisBacterial ProteinsCarbonCytochromes cDisulfidesOxidoreductasesCytochrome cEpsPathogenesisXanthomonas campestris

Identifiers

PMID28655353
PMCPMC5488342
OpenAlexW2729281848

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.