ArticleNucleic acids research2017
Highly efficient preparation of single-stranded DNA rings by T4 ligase at abnormally low Mg(II) concentration.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 53 citations in OpenAlex.
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- Site-Specifically Modified Circular Ribonucleic Acid Serves as Multitarget miRNA Sponge with Low Immunogenicity.Journal of the American Chemical Society · 2026Article
- A rapid and ultrasensitive CRISPR-Cas12a assay for clinical detection of pathogens and mutations.Analytical and bioanalytical chemistry · 2026Article
- Spatial transcriptomic imaging of an intact organism using volumetric DNA microscopy.Nature biotechnology · 2026Article
- MYC drives left-handed Z-DNA formation to shape gene expression.Nature communications · 2025Article
- Click DNA ligation with deoxyribozyme.Nucleic acids research · 2025Article
- Evolution of multivalent supramolecular assemblies of aptamers with target-defined spatial organization.Nature nanotechnology · 2025Article
- Facile Splint-Free Circularization of ssDNA with T4 DNA Ligase by Redesigning the Linear Substrate to Form an Intramolecular Dynamic Nick.Biomolecules · 2024Article
- Reversible strain-promoted DNA polymerization.Science advances · 2024Article
- Structure ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Direct dsRNA preparation by promoter-free RCT and RNase H cleavage using one circular dsDNA template with a mismatched bubble.RNA (New York, N.Y.) · 2023Article
- Engineering a Ligase Binding DNA Aptamer into a Templating DNA Scaffold to Guide the Selective Synthesis of Circular DNAzymes and DNA Aptamers.Journal of the American Chemical Society · 2023Article
- Nanorings to Probe Mechanical Stress of Single-Stranded DNA Mediated by the DNA Duplex.International journal of molecular sciences · 2022Article
- Construction of ssDNA-Attached LR-Chimera Involving Z-DNA for ZBP1 Binding Analysis.Molecules (Basel, Switzerland) · 2022Article
- Anti-Interference Detection ofFoods (Basel, Switzerland) · 2022Article
- Quantitative methods to study helicase, DNA polymerase, and exonuclease coupling during DNA replication.Methods in enzymology · 2022Article
- Rolling Circle Amplification as a Universal Method for the Analysis of a Wide Range of Biological Targets.Russian journal of bioorganic chemistry · 2021Article
- Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand.Nucleic acids research · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preparation of large amount of single-stranded circular DNA in high selectivity is crucial for further developments of nanotechnology and other DNA sciences. Herein, a simple but practically useful methodology to prepare DNA rings has been presented. One of the essential factors is to use highly diluted T4 ligase buffer for ligase reactions. This strategy is based on our unexpected finding that, in diluted T4 buffers, intermolecular polymerization of DNA fragments is greatly suppressed with respect to their intramolecular cyclization. This promotion of cyclization is attributable to abnormally low concentration of Mg2+ ion (0.5-1.0 mM) but not ATP in the media for T4 ligase reactions. The second essential factor is to add DNA substrate intermittently to the mixture and maintain its temporal concentration low. By combining these two factors, single-stranded DNA rings of various sizes (31-74 nt) were obtained in high selectivity (89 mol% for 66-nt DNA) and in satisfactorily high productivity (∼0.2 mg/ml). A linear 72-nt DNA was converted to the corresponding DNA ring in nearly 100% selectivity. The superiority of this new method was further substantiated by the fact that small-sized DNA rings (31-42 nt), which were otherwise hardly obtainable, were successfully prepared in reasonable yields.
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