ArticlePloS one2017
Influence of the way of reporting alpha-Amylase values in saliva in different naturalistic situations: A pilot study.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Effect of Acute Hypohydration on Indicators of Glycemic Regulation, Appetite, Metabolism and Stress: A Systematic Review and Meta-Analysis.Nutrients · 2020Pooled it
- Alpha-Amylase Activity in Feline Saliva: An Analytical Validation of an Automated Assay for Its Measurement and a Pilot Study on Its Changes Following Acute Stress and Due to Urinary Tract Pathologies.Animals : an open access journal from MDPI · 2025Article
- Welfare Assessment in Pigs Using the Salivary Proteome.Animals : an open access journal from MDPI · 2024Review
- Extracellular DNA and Markers of Neutrophil Extracellular Traps in Saliva from Patients with Periodontitis-A Case-Control Study.Journal of clinical medicine · 2024Article
- Potential use of salivary TNF-α as a vaccine-induced pain biomarker in people with cerebral palsy and communication disorders.PloS one · 2024Article
- Salivary alpha-amylase: A marker of stress in gynecological residents during a shoulder dystocia simulation scenario.PloS one · 2024Article
- Beyond the Bottle: Exploring Health-Promoting Compounds in Wine and Wine-Related Products-Extraction, Detection, Quantification, Aroma Properties, and Terroir Effects.Foods (Basel, Switzerland) · 2023Review
- Changes in Calprotectin (S100A8-A9) and Aldolase in the Saliva of Horses with Equine Gastric Ulcer Syndrome.Animals : an open access journal from MDPI · 2023Article
- Changes in the Saliva Proteome of Pigs with Diarrhoea Caused byProteomes · 2023Article
- Exploring the Impact of α-Amylase Enzyme Activity and pH on Flavor Perception of Alcoholic Drinks.Foods (Basel, Switzerland) · 2023Article
- Effects of filtration and alpha-amylase depletion on salivary biochemical composition measurements.PloS one · 2023Article
- Tissue-specific assessment of oxidative status: Wild boar as a case study.Frontiers in veterinary science · 2023Article
- Salivary secretory leukocyte protease inhibitor levels in patients with stage 3 grade C periodontitis: a comparative cross-sectional study.Scientific reports · 2022Article
- Basics for the potential use of saliva to evaluate stress, inflammation, immune system, and redox homeostasis in pigs.BMC veterinary research · 2022Review
- Salivary Ferritin Changes in Patients with COVID-19.International journal of environmental research and public health · 2021Article
- Changes in salivary biomarkers of oxidative status in calves at weaning and grouping.BMC veterinary research · 2021Article
- Saliva: What Dental Practitioners Should Know about the Role of This Biofluid in the Transmission and Diagnostic of SARS-CoV-2.Medicina (Kaunas, Lithuania) · 2021Review
- Article
- Salivary biomarkers in breast cancer: a cross-sectional study.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2021Article
- Stress-Related Responses to Alternations between Repetitive Physical Work and Cognitive Tasks of Different Difficulties.International journal of environmental research and public health · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
The objective of this pilot study was to compare the different ways of measuring salivary alpha-amylase (sAA, enzymatic vs. concentration) and to evaluate the influence that the different ways of reporting the results can have in sAA interpretation. For this purpose, sAA was measured by direct quantification and also by an enzymatic assay in three different naturalistic situations, a physical stressor (situation 1) and two mental stressors of different intensity (situations 2 and 3). The results were expressed in three different ways (without correction, multiplied by flow rate and divided by protein concentration). sAA concentration and activity increased just after situations 1 and 3. When values were multiplied by the flow rate, significant changes after situation 1 were detected only for sAA activity but not for sAA concentration, being these changes of lower significance and magnitude that those observed for sAA activity without any correction. In addition, a significant increase in sAA activity was found at T+15 in situation 2. In situation 3 the significant decrease in sAA at T+15 disappeared. When values were divided by protein concentration, there were no significant changes in situations 1 or 3, but a decrease in situation 2 at T+0 and an increase at T+15. sAA activity and concentration showed a significant correlation in all situations. This pilot study points out that the way of expressing sAA can influence the results obtained in different stress models and also their interpretation. Therefore, how sAA is reported and the factors involved in the different ways of expressing sAA, should be taken into consideration for an objective interpretation of sAA values.
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