ReviewAnnual review of biochemistry2017
Metabolite Measurement: Pitfalls to Avoid and Practices to Follow.
Review in Annual review of biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 220 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
220 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Metabolomic characteristics and related pathways in patients with different severity of COVID-19: a systematic review and meta-analysis.Journal of global health · 2025Pooled it
- An integrated meta-analysis of peripheral blood metabolites and biological functions in major depressive disorder.Molecular psychiatry · 2021Pooled it
- The metabolic signature of cardiorespiratory fitness: a protocol for a systematic review and meta-analysis.BMJ open sport & exercise medicine · 2021Pooled it
- Real-time NMR analysis of glutamine metabolism for screening anti-cancer agents in living cells.Magnetic resonance letters · 2027Article
- Targeted detection of amino acid profiles in pleural fluid and serum by liquid chromatography-tandem mass spectrometry for estimating the risks of four aetiologies of pleural effusion: a proof-of-concept study.Annals of medicine · 2026Article
- Sub-3 μm porous graphitic carbon for highly polar metabolomics: orthogonal selectivity and comparison with HILIC and reversed-phase chromatography.Analytical and bioanalytical chemistry · 2026Article
- Glutamine from plasma-like medium fuels nitrogen metabolism and influences proliferation in Leptospira interrogans.PLoS pathogens · 2026Article
- From Sensation to Action: Neuroplasticity, Cognitive-Motor Training, and Emerging Biomarkers of Adaptation.Brain sciences · 2026Review
- Unveiling the anticancer potential of medicinal plants: metabolomics and analytical tools in phytomedicine.Molecular diversity · 2026Review
- Advanced technologies of single-cell metabolomics unveiling cellular metabolic heterogeneity for biological and biomedical research.Journal of food and drug analysis · 2026Review
- The Role of Kynurenine and 5-Hydroxytryptophan in Modulating Microbiota and Their Implications in Exudative Age-Related Macular Degeneration.Diagnostics (Basel, Switzerland) · 2026Article
- Article
- Decoding cancer across scales with metabolomics.Nature reviews. Cancer · 2026Review
- The chemistry of the nitrate-nitrite-nitric oxide pathway: regulating muscle oxygenation and exercise performance.RSC advances · 2026Review
- Metabolic reprogramming of tomato roots during rhizobacteria-mediated defense againstFrontiers in plant science · 2026Article
- Evaluation of LC/MS Methods for Hydrophilic Metabolites to Enable Integration of Human Blood Metabolome Data.Mass spectrometry (Tokyo, Japan) · 2026Article
- HUBMet: an integrative database and analytical platform for human blood metabolites and metabolite-protein associations.Genome biology · 2025Article
- mzLearn as a data-driven LC/MS signal detection algorithm that enables pre-trained generative models for untargeted metabolomics.Communications chemistry · 2025Article
- SIMBA-GNN: mechanistic graph learning for microbiome prediction.NPJ systems biology and applications · 2025Article
- Untargeted Metabolomics Reveals Distinct Soil Metabolic Profiles Across Land Management Practices.Metabolites · 2025Article
160 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Metabolites are the small biological molecules involved in energy conversion and biosynthesis. Studying metabolism is inherently challenging due to metabolites' reactivity, structural diversity, and broad concentration range. Herein, we review the common pitfalls encountered in metabolomics and provide concrete guidelines for obtaining accurate metabolite measurements, focusing on water-soluble primary metabolites. We show how seemingly straightforward sample preparation methods can introduce systematic errors (e.g., owing to interconversion among metabolites) and how proper selection of quenching solvent (e.g., acidic acetonitrile:methanol:water) can mitigate such problems. We discuss the specific strengths, pitfalls, and best practices for each common analytical platform: liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR), and enzyme assays. Together this information provides a pragmatic knowledge base for carrying out biologically informative metabolite measurements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.