Evidence map›Paper›PMID 28654088›Full record

ArticleScientific data2017

Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source.

Hemanth K N Reddy, Chun Hong Yoon, Andrew Aquila, Salah Awel, Kartik Ayyer, Anton Barty, Peter Berntsen, Johan Bielecki, Sergey Bobkov, Maximilian Bucher and 35 more

Abstract readDataset
In one paragraph

Article in Scientific data, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

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  12. Journal of applied crystallography · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

45 authors.

Hemanth K N ReddyLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Chun Hong YoonSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Andrew AquilaSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Salah AwelCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Kartik AyyerCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.ORCID 0000-0002-6881-564X
Anton BartyCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Peter BerntsenAustralian Research Council Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia.ORCID 0000-0003-2136-4105
Johan BieleckiLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Sergey BobkovNational Research Centre 'Kurchatov Institute', Akademika Kurchatova pl. 1, 123182 Moscow, Russia.
Maximilian BucherSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Gabriella A CariniSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Sebastian CarronSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Henry ChapmanCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.ORCID 0000-0002-4655-1743
Benedikt DaurerLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.ORCID 0000-0002-1887-7551
Hasan DeMirciSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.ORCID 0000-0002-9135-5397
Tomas EkebergLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Petra FrommeBiodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe, Arizona 85287-5001, USA.
Janos HajduLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Max Felix HankeLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Philip HartSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Brenda G HogueBiodesign Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe, Arizona 85287-5001, USA.
Ahmad HosseinizadehDepartment of Physics, University of Wisconsin Milwaukee, KIRC, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Yoonhee KimDepartment of Physics, Pohang University of Science and Technology, Pohang 37673, Korea.
Richard A KirianDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Ruslan P KurtaEuropean X-ray Free Electron Laser GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.
Daniel S D LarssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
N Duane LohDepartment of Physics, National University of Singapore Blk S12, 2 Science Drive 3, Singapore 117551.
Filipe R N C MaiaLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Adrian P MancusoEuropean X-ray Free Electron Laser GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.
Kerstin MühligLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Anna MunkeLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.ORCID 0000-0002-5510-2245
Daewoong NamDepartment of Physics, Pohang University of Science and Technology, Pohang 37673, Korea.
Carl NettelbladLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Abbas OurmazdDepartment of Physics, University of Wisconsin Milwaukee, KIRC, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.ORCID 0000-0001-9946-3889
Max RoseDeutsches Elektronen-Synchrotron DESY, Notkestraße 85, D-22607 Hamburg, Germany.
Peter SchwanderDepartment of Physics, University of Wisconsin Milwaukee, KIRC, 3135 N. Maryland Ave, Milwaukee, Wisconsin 53211, USA.
Marvin SeibertLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Jonas A SellbergLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.ORCID 0000-0003-2793-5052
Changyong SongDepartment of Physics, Pohang University of Science and Technology, Pohang 37673, Korea.
John C H SpenceDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Martin SvendaLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Gijs Van der SchotLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-75124, Sweden.
Ivan A VartanyantsDeutsches Elektronen-Synchrotron DESY, Notkestraße 85, D-22607 Hamburg, Germany.
Garth J WilliamsNSLS-II, Brookhaven National Laboratory, Upton, New York 11873, USA.
P Lourdu XavierCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.

Funding

Femtosecond nano-crystallography of membrane proteinsR01GM095583 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2023
$4.3M
NIGMS NIH HHS R01 GM095583
6 · The paper itself

Abstract

Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.

Indexed as

ColiphagesAlgorithmsMolecular StructureX-Ray Diffraction

Identifiers

PMID28654088
PMCPMC5501160

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