ArticleJournal of visualized experiments : JoVE2017
Clock Scan Protocol for Image Analysis: ImageJ Plugins.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
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- B cell adapter for PI 3-kinase (BCAP) coordinates antigen internalization and trafficking through the B cell receptor.Science advances · 2024Article
- Dynamic interaction of REEP5-MFN1/2 enables mitochondrial hitchhiking on tubular ER.The Journal of cell biology · 2024Article
- LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance.Nucleic acids research · 2023Article
- In vivo inhibition of nuclear ACE2 translocation protects against SARS-CoV-2 replication and lung damage through epigenetic imprinting.Nature communications · 2023Article
- Independent control over cell patterning and adhesion on hydrogel substrates for tissue interface mechanobiology.iScience · 2023Article
- Article
- Autophagy impairment as a key feature for acetaminophen-induced ototoxicity.Cell death & disease · 2021Article
- Ecm29-Dependent Proteasome Localization Regulates Cytoskeleton Remodeling at the Immune Synapse.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The clock scan protocol for image analysis is an efficient tool to quantify the average pixel intensity within, at the border, and outside (background) a closed or segmented convex-shaped region of interest, leading to the generation of an averaged integral radial pixel-intensity profile. This protocol was originally developed in 2006, as a visual basic 6 script, but as such, it had limited distribution. To address this problem and to join similar recent efforts by others, we converted the original clock scan protocol code into two Java-based plugins compatible with NIH-sponsored and freely available image analysis programs like ImageJ or Fiji ImageJ. Furthermore, these plugins have several new functions, further expanding the range of capabilities of the original protocol, such as analysis of multiple regions of interest and image stacks. The latter feature of the program is especially useful in applications in which it is important to determine changes related to time and location. Thus, the clock scan analysis of stacks of biological images may potentially be applied to spreading of Na
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