ArticleJournal of visualized experiments : JoVE2017
Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Locomotion-dependent auditory gating to the parietal cortex guides multisensory decisions.Nature communications · 2025Article
- Restoration of excitation/inhibition balance enhances neuronal signal-to-noise ratio and rescues social deficits in autism-associatedbioRxiv : the preprint server for biology · 2025Article
- Endocannabinoid-mediated rescue of somatosensory cortex activity, plasticity and related behaviors following an early in life concussion.bioRxiv : the preprint server for biology · 2024Article
- Advances in cellular resolution microscopy for brain imaging in rats.Neurophotonics · 2023Review
- Noradrenergic Signaling in Astrocytes Influences Mammalian Sleep Homeostasis.Clocks & sleep · 2022Article
- Are the Anterior and Mid-Cingulate Cortices Distinct in Rodents?Frontiers in neuroanatomy · 2022Article
- Head-mounted microendoscopic calcium imaging in dorsal premotor cortex of behaving rhesus macaque.Cell reports · 2021Article
- Location Matters: Navigating Regional Heterogeneity of the Neurovascular Unit.Frontiers in cellular neuroscience · 2021Review
- A Role for Astroglial Calcium in Mammalian Sleep and Sleep Regulation.Current biology : CB · 2020Article
- Diving into the brain: deep-brain imaging techniques in conscious animals.The Journal of endocrinology · 2020Review
- Multiphoton intravital microscopy in small animals: motion artefact challenges and technical solutions.Journal of microscopy · 2020Review
- Deciphering Brain Function by Miniaturized Fluorescence Microscopy in Freely Behaving Animals.Frontiers in neuroscience · 2020Review
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
In vivo circuit and cellular level functional imaging is a critical tool for understanding the brain in action. High resolution imaging of mouse cortical neurons with two-photon microscopy has provided unique insights into cortical structure, function and plasticity. However, these studies are limited to head fixed animals, greatly reducing the behavioral complexity available for study. In this paper, we describe a procedure for performing chronic fluorescence microscopy with cellular-resolution across multiple cortical layers in freely behaving mice. We used an integrated miniaturized fluorescence microscope paired with an implanted prism probe to simultaneously visualize and record the calcium dynamics of hundreds of neurons across multiple layers of the somatosensory cortex as the mouse engaged in a novel object exploration task, over several days. This technique can be adapted to other brain regions in different animal species for other behavioral paradigms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.