Evidence map›Paper›PMID 28653216›Full record

ArticleJournal of biomolecular NMR2017

NMR characterization of HtpG, the E. coli Hsp90, using sparse labeling with

Kari Pederson, Gordon R Chalmers, Qi Gao, Daniel Elnatan, Theresa A Ramelot, Li-Chung Ma, Gaetano T Montelione, Michael A Kennedy, David A Agard, James H Prestegard

Abstract read
In one paragraph

Article in Journal of biomolecular NMR, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 19 citations in OpenAlex.

  1. A random fractionalMagnetic resonance letters · 2026
    Article
  2. AMIGO - Guided assignment ofJournal of biomolecular NMR · 2026
    Article
  3. Article
  4. Article
  5. Hsp90, a team player in protein quality control and the stress response in bacteria.Microbiology and molecular biology reviews : MMBR · 2024
    Review
  6. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Kari PedersonComplex Carbohydrate Research Center, University of Georgia, Athens, USA.
Gordon R ChalmersComplex Carbohydrate Research Center, University of Georgia, Athens, USA.
Qi GaoComplex Carbohydrate Research Center, University of Georgia, Athens, USA.
Daniel ElnatanDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, USA.
Theresa A RamelotDepartment of Chemistry and Biochemistry, Miami University, Oxford, USA.
Li-Chung MaDepartment of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, The State University of New Jersey, Piscataway, USA.
Gaetano T MontelioneDepartment of Molecular Biology and Biochemistry, Center for Advanced Biotechnology and Medicine, The State University of New Jersey, Piscataway, USA.
Michael A KennedyDepartment of Chemistry and Biochemistry, Miami University, Oxford, USA.
David A AgardDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, USA.
James H PrestegardComplex Carbohydrate Research Center, University of Georgia, Athens, USA. jpresteg@ccrc.uga.edu.ORCID http://orcid.org/0000-0002-1602-4790
University of Georgia · USHoward Hughes Medical Institute · USMiami University · USRutgers, The State University of New Jersey · US

Funding

University of Georgia ProjectU54GM094597 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI MONTELIONE, GAETANO T · 2010 to 2014
$36.9M
TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI BOONS, GEERT-JAN · 2012 to 2019
$14.5M
Resource for Biocomputing Visualization and InformaticsP41GM103311 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2012 to 2017
$8.2M
NMR of Cell Surface OligosaccharidesR01GM033225 · NIGMS · YALE UNIVERSITY · PI PRESTEGARD, JAMES H. · 1985 to 2021
$4.6M
Howard Hughes Medical InstituteNIGMS NIH HHS P41 GM103311NIGMS NIH HHS P41 GM103390NIGMS NIH HHS R01 GM033225NIGMS NIH HHS U54 GM094597
6 · The paper itself

Abstract

A strategy for acquiring structural information from sparsely isotopically labeled large proteins is illustrated with an application to the E. coli heat-shock protein, HtpG (high temperature protein G), a 145 kDa dimer. It uses

Indexed as

Staining and LabelingAlanineCarbon IsotopesEscherichia coli ProteinsHSP90 Heat-Shock ProteinsMethylationModels, MolecularNuclear Magnetic Resonance, BiomolecularProtein DomainsProtein Structure, SecondaryAlanineCarbon IsotopesEscherichia coli ProteinsHSP90 Heat-Shock ProteinsHtpG protein, E coli1H–13C Ala methyl RDCsAssignment programHeat-shock proteinHsp90HtpGMethyl-TROSYPerdeuterationProtein structureResonance assignmentsSparse labeling

Identifiers

PMID28653216
PMCPMC5546222
OpenAlexW2637412536

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.