ArticleCytotechnology2017
Spreading, proliferation and differentiation of human dental pulp stem cells on chitosan scaffolds immobilized with RGD or fibronectin.
Article in Cytotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Cytotoxicity, DNA damage, and migration of SHEDs exposed to bioactive pulp capping materials: an in vitro study.BMC oral health · 2026Article
- 3D Printing of Chitosan Scaffolds and Films with Varying Roughness for Cultivation of Human Retinal Progenitor Cells.ACS omega · 2025Article
- Advancements in Chitosan-Based Scaffolds for Chondrogenic Differentiation and Knee Cartilage Regeneration: Current Trends and Future Perspectives.Bioengineering (Basel, Switzerland) · 2025Review
- Characterization of Fibronectin-Adherent, Non-Fibronectin-Adherent, and Explant-Derived Human Dental Pulp Stem Cell Populations.Dentistry journal · 2025Article
- A Thermally Stable Recombinant Human Fibronectin Peptide-Fused Protein (rhFN3C) for Faster Aphthous Ulcer (AU) Healing.Bioengineering (Basel, Switzerland) · 2023Article
- Photobiomodulation Therapy and Pulp-Regenerative Endodontics: A Narrative Review.Bioengineering (Basel, Switzerland) · 2023Review
- Review
- Fibronectin and Its Applications in Dentistry and Periodontics: A Cell Behaviour Conditioner.Cureus · 2022Review
- Cytocompatibility and bioactivity of calcium hydroxide-containing nanofiber scaffolds loaded with fibronectin for dentin tissue engineering.Clinical oral investigations · 2022Article
- Scaffolds from medical grade chitosan: A good choice for 3D cultivation of mesenchymal stem cells.Turkish journal of biology = Turk biyoloji dergisi · 2022Article
- Which experimental models and explorations to use in regenerative endodontics? A comprehensive review on standard practices.Molecular biology reports · 2021Review
- Article
- Effect of Magnesium on Dentinogenesis of Human Dental Pulp Cells.International journal of biomaterials · 2021Article
- Stiffness Regulates the Morphology, Adhesion, Proliferation, and Osteogenic Differentiation of Maxillary Schneiderian Sinus Membrane-Derived Stem Cells.Stem cells international · 2021Article
- Physical and Biological Properties of a Chitosan Hydrogel Scaffold Associated to Photobiomodulation Therapy for Dental Pulp Regeneration: AnBioMed research international · 2021Article
- Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications-A Review.Polymers · 2020Review
- Synergistic potential of 1α,25-dihydroxyvitamin D3 and calcium-aluminate-chitosan scaffolds with dental pulp cells.Clinical oral investigations · 2020Article
- Boron mediated 2D and 3D cultures of adipose derived mesenchymal stem cells.Cytotechnology · 2019Article
- Quantifying extracellular matrix turnover in human lung scaffold cultures.Scientific reports · 2018Article
- Extracellular matrix stiffness controls osteogenic differentiation of mesenchymal stem cells mediated by integrin α5.Stem cell research & therapy · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nowadays, human dental pulp stem cells (hDPSCs) became more attractive for therapeutic purposes because of their high proliferation and differentiation potential. Thus, coupling the desired cellular characteristics of hDPSCs with good biomaterial properties of the chitosan scaffolds provide an interesting approach for tissue engineering applications. On the other hand, scaffold surface modification is also needed to promote stem cell adhesion since chitosan lacks adhesion motifs to support direct cell anchorage. In this study, hDPSCs were isolated from third molars of healthy female individuals (aged 16-25) with enzymatic digestion. For cell culture studies, the chitosan scaffolds which have approximately 9 mm diameter and 2 mm thickness with interconnected structure were prepared by freeze-drying. To support cellular attachment the scaffolds were covalently immobilized with either RGD (arginine-glycine-aspartic acid) or fibronectin (Fn) molecules. Cells were seeded on chitosan scaffolds with or without immobilized RGD and fibronectin. Cell attachment, spreading, adhesion behaviors and proliferation capacity were examined by scanning electron microscopy, immunofluorescence staining and PrestoBlue
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.