ArticleProceedings of the National Academy of Sciences of the United States of America2017
DNA cytoskeleton for stabilizing artificial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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Who cites it
55 citing papers in PubMed, 151 citations in OpenAlex.
- Elucidating the Reciprocal Interplay between Supramolecular Polymer Cytoskeletons and Fuel-Dependent Synthetic Cells.Journal of the American Chemical Society · 2026Article
- A synthetic cell microreactor with two types of interacting dynamic DNA-based pores.Nature chemistry · 2026Article
- Mechanically Programmable DNA Hydrogel Microparticles for 3D Cellular Systems.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Programming Nonlinear Interfacial Mechanics of Synthetic Cells: Lipid Geometry and DNA Nanostructures.Small science · 2026Article
- Vesicle-Templated Self-Assembly of Programmable Freestanding Multi-μm DNA Shells.Nano letters · 2026Article
- Peptide-based artificial cytoskeleton enhances colocalized cascade reactions in cell-like microreactors.Chemical science · 2026Article
- Nature-Inspired Enzymatic Cascades: Emerging Strategies for Sustainable Chemistry.Molecules (Basel, Switzerland) · 2026Review
- Stimuli-Responsive Chemical Systems That Mimic Biological Dynamics.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- DNA Origami and Its Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Cytoskeleton-functionalized synthetic cells with life-like mechanical features and regulated membrane dynamicity.Nature chemistry · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Morphology remodelling and membrane channel formation in synthetic cells via reconfigurable DNA nanorafts.Nature materials · 2025Article
- DNA-empowered synthetic cells as minimalistic life forms.Nature reviews. Chemistry · 2024Review
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
- Dynamic control of DNA condensation.Nature communications · 2024Article
- Functional metal-phenolic cortical cytoskeleton for artificial cells.Science advances · 2024Article
- Protocells Capable of Generating a Cytoskeleton-like Structure from intracellular Membrane-active Artificial Organelles.Advanced functional materials · 2023Article
- Engineering DNA-based cytoskeletons for synthetic cells.Interface focus · 2023Review
- Synthetic Cell Armor Made of DNA Origami.Nano letters · 2023Article
- Charge controlled interactions between DNA-modified silica nanoparticles and fluorosurfactants in microfluidic water-in-oil droplets.Nanoscale advances · 2023Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-sized liposomes and droplets coated with lipid layers have been used as platforms for understanding live cells, constructing artificial cells, and implementing functional biomedical tools such as biosensing platforms and drug delivery systems. However, these systems are very fragile, which results from the absence of cytoskeletons in these systems. Here, we construct an artificial cytoskeleton using DNA nanostructures. The designed DNA oligomers form a Y-shaped nanostructure and connect to each other with their complementary sticky ends to form networks. To undercoat lipid membranes with this DNA network, we used cationic lipids that attract negatively charged DNA. By encapsulating the DNA into the droplets, we successfully created a DNA shell underneath the membrane. The DNA shells increased interfacial tension, elastic modulus, and shear modulus of the droplet surface, consequently stabilizing the lipid droplets. Such drastic changes in stability were detected only when the DNA shell was in the gel phase. Furthermore, we demonstrate that liposomes with the DNA gel shell are substantially tolerant against outer osmotic shock. These results clearly show the DNA gel shell is a stabilizer of the lipid membrane akin to the cytoskeleton in live cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.