ArticleMethods (San Diego, Calif.)2017
Single molecule fluorescence in situ hybridisation for quantitating post-transcriptional regulation in Drosophila brains.
Article in Methods (San Diego, Calif.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Detection of Nora virus in infectedFly · 2026Article
- Transcriptional Bursting in Pluripotent Stem Cells.Biology · 2026Review
- Hunger Recruits a Parallel Circuit Encoding Alcohol Reward.bioRxiv : the preprint server for biology · 2025Article
- Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization.Journal of visualized experiments : JoVE · 2025Article
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- Systematic analysis of YFP traps reveals common mRNA/protein discordance in neural tissues.The Journal of cell biology · 2023Article
- Buffered EGFR signaling regulated by spitz-to-argos expression ratio is a critical factor for patterning the Drosophila eye.PLoS genetics · 2023Article
- Illuminati: a form of gene expression plasticity in Drosophila neural stem cells.Development (Cambridge, England) · 2022Article
- The long noncoding RNAScience advances · 2022Article
- Post-transcriptional regulation of transcription factor codes in immature neurons drives neuronal diversity.Cell reports · 2022Article
- An expanded toolkit foreLife · 2022Article
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- The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis.Cell reports · 2021Article
- Hypoxic and pharmacological activation of HIF inhibits SARS-CoV-2 infection of lung epithelial cells.Cell reports · 2021Article
- Selective dendritic localization of mRNA ineLife · 2021Article
- Quantifying the Central Dogma in the p53 Pathway in Live Single Cells.Cell systems · 2020Article
- Neuronal upregulation of Prospero protein is driven by alternative mRNA polyadenylation and Syncrip-mediated mRNA stabilisation.Biology open · 2020Article
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9 authors.
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Abstract
RNA in situ hybridization is a powerful method to investigate post-transcriptional regulation, but analysis of intracellular mRNA distributions in thick, complex tissues like the brain poses significant challenges. Here, we describe the application of single-molecule fluorescent in situ hybridization (smFISH) to quantitate primary nascent transcription and post-transcriptional regulation in whole-mount Drosophila larval and adult brains. Combining immunofluorescence and smFISH probes for different regions of a single gene, i.e., exons, 3'UTR, and introns, we show examples of a gene that is regulated post-transcriptionally and one that is regulated at the level of transcription. Our simple and rapid protocol can be used to co-visualise a variety of different transcripts and proteins in neuronal stem cells as well as deep brain structures such as mushroom body neuropils, using conventional confocal microscopy. Finally, we introduce the use of smFISH as a sensitive alternative to immunofluorescence for labelling specific neural stem cell populations in the brain.
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