ArticlePLoS neglected tropical diseases2017
A study of ticks and tick-borne livestock pathogens in Pakistan.
Article in PLoS neglected tropical diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 2 of them syntheses that pooled it.
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Who cites it
109 citing papers in PubMed, 2 syntheses or guidelines pooled it, 261 citations in OpenAlex.
- Tick-Borne Diseases in Sub-Saharan Africa: A Systematic Review of Pathogens, Research Focus, and Implications for Public Health.Pathogens (Basel, Switzerland) · 2024Pooled it
- Geographical epidemiology of Hyalomma anatolicum and Rhipicephalus microplus in Pakistan: A systematic review.PloS one · 2024Pooled it
- First molecular identification of Dermacentor montanus and new locality record of Argas hermanni from Hindu Kush and Himalayan ranges.Experimental & applied acarology · 2026Article
- Article
- Metagenomic and serological evidence of emerging tick-borne viruses in livestock, humans, and rats in Pakistan.Virologica Sinica · 2026Article
- Q fever in Kazakhstan: seroepidemiology, molecular evidence, and spatial distribution across livestock and ticks.BMC veterinary research · 2026Article
- Diversity, Host Attachment Preferences and Role of Tick Rhipicephalus microplus in the Transmission of Bartonella spp. and Rickettsia spp. in Cattle From Southern Benin.Veterinary medicine and science · 2026Article
- Genetic diversity and co-circulation of tick-borne pathogens in Haemaphysalis spp. from Pakistan.Parasitology research · 2026Article
- Molecular-based detection of Ehrlichia spp. in stray dogs-infesting Rhipicephalus ticks in high-altitude of northern Pakistan.PloS one · 2026Article
- Co-Circulation of Tick-BorneViruses · 2025Article
- Metagenomic Profile of Bacterial Communities ofPathogens (Basel, Switzerland) · 2025Article
- Effects of Tick Infestation on Milk Yield, Blood Biochemistry, Hematology, and the Overall Health of Dairy Cows.Pathogens (Basel, Switzerland) · 2025Article
- Distribution ofParasite epidemiology and control · 2025Review
- Morphological and genetic discrepancy of Rhipicephalus sp. (Acari: Ixodidae) collected from the ricefield rat, Rattus argentiventer, in Peninsular Malaysia.Experimental & applied acarology · 2025Article
- High Diversity and Low Coinfections of Pathogens in Ticks from Ruminants in Pakistan.Microorganisms · 2025Article
- Molecular detection and characterization of spotted fever groupParasitology · 2025Article
- Fourteen anti-tick vaccine targets are variably conserved in cattle fever ticks.Parasites & vectors · 2025Article
- Tick Control Strategies: Critical Insights into Chemical, Biological, Physical, and Integrated Approaches for Effective Hard Tick Management.Veterinary sciences · 2025Review
- Establishment of Pathogen-FreeVeterinary sciences · 2025Article
- Molecular Profiling of Ticks and Associated Pathogens: First Report ofTransboundary and emerging diseases · 2025Article
49 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 2 countries.
Funding
Abstract
backgroundAs obligate blood-feeding arthropods, ticks transmit pathogens to humans and domestic animals more often than other arthropod vectors. Livestock farming plays a vital role in the rural economy of Pakistan, and tick infestation causes serious problems with it. However, research on tick species diversity and tick-borne pathogens has rarely been conducted in Pakistan. In this study, a systematic investigation of the tick species infesting livestock in different ecological regions of Pakistan was conducted to determine the microbiome and pathobiome diversity in the indigenous ticks. METHODOLOGY/PRINCIPAL
findingsA total of 3,866 tick specimens were morphologically identified as 19 different tick species representing three important hard ticks, Rhipicephalus, Haemaphysalis and Hyalomma, and two soft ticks, Ornithodorus and Argas. The bacterial diversity across these tick species was assessed by bacterial 16S rRNA gene sequencing using a 454-sequencing platform on 10 of the different tick species infesting livestock. The notable genera detected include Ralstonia, Clostridium, Staphylococcus, Rickettsia, Lactococcus, Lactobacillus, Corynebacterium, Enterobacter, and Enterococcus. A survey of Spotted fever group rickettsia from 514 samples from the 13 different tick species generated rickettsial-specific amplicons in 10% (54) of total ticks tested. Only three tick species Rhipicephalus microplus, Hyalomma anatolicum, and H. dromedarii had evidence of infection with "Candidatus Rickettsia amblyommii" a result further verified using a rompB gene-specific quantitative PCR (qPCR) assay. The Hyalomma ticks also tested positive for the piroplasm, Theileria annulata, using a qPCR assay. CONCLUSIONS/SIGNIFICANCE: This study provides information about tick diversity in Pakistan, and pathogenic bacteria in different tick species. Our results showed evidence for Candidatus R. amblyommii infection in Rhipicephalus microplus, H. anatolicum, and H. dromedarii ticks, which also carried T. annulata.
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Registered trials
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