ArticleNature methods2017
A DNA origami platform for quantifying protein copy number in super-resolution.
Article in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 119 citations in OpenAlex.
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- Enhanced spatial clustering of single-molecule localizations with graph neural networks.Nature communications · 2025Article
- GRIN2B disease-associated mutations disrupt the function of BK channels and NMDA receptor signaling nanodomains.The Journal of general physiology · 2025Article
- Revealing the biophysics of lamina-associated domain formation by integrating theoretical modeling and high-resolution imaging.Nature communications · 2025Article
- Polymer model integrates imaging and sequencing to reveal how nanoscale heterochromatin domains influence gene expression.Nature communications · 2025Article
- Wnt signaling modulates mechanotransduction in the epidermis to drive hair follicle regeneration.Science advances · 2025Article
- Super-Resolution Microscopy as a Versatile Tool in Probing Molecular Assembly.International journal of molecular sciences · 2024Review
- Quantification of absolute labeling efficiency at the single-protein level.Nature methods · 2024Article
- Piggybacking functionalized DNA nanostructures into live-cell nuclei.Science advances · 2024Article
- Piggybacking functionalized DNA nanostructures into live cell nuclei.bioRxiv : the preprint server for biology · 2024Article
- Interplay between stochastic enzyme activity and microtubule stability drives detyrosination enrichment on microtubule subsets.Current biology : CB · 2023Article
- Super-resolution microscopy reveals the nanoscale cluster architecture of the DEK protein cancer biomarker.iScience · 2023Article
- Nucleic Acid Probes for Single-Molecule Localization Imaging of Cellular Biomolecules.Chemical & biomedical imaging · 2023Review
- Article
- Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission.Journal of cell science · 2023Article
- Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues.Nature biomedical engineering · 2023Article
- Functionalizing DNA origami to investigate and interact with biological systems.Nature reviews. Materials · 2023Article
- A novel and robust method for counting components within bio-molecular complexes using fluorescence microscopy and statistical modelling.Scientific reports · 2022Article
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
- Technological advances in super-resolution microscopy to study cellular processes.Molecular cell · 2022Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Single-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here we show that DNA origami, in combination with GFP antibodies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy number in cellular contexts using super-resolution microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.