ArticleNature biotechnology2017
A calcium- and light-gated switch to induce gene expression in activated neurons.
Article in Nature biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
94 citing papers in PubMed, 163 citations in OpenAlex.
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- A bioluminescent activity dependent platform, BLADe, for converting intracellular activity to photoreceptor activation.Scientific reports · 2026Article
- Sensing and Reprogramming Surface Receptor Activation With Synthetic Transcriptional Circuits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cell type-specific barcoding reveals the single-neuron projectional architecture of the mouse midbrain dopaminergic system.bioRxiv : the preprint server for biology · 2026Article
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- Photochemical nanomotors reverse anxiety- and depressive-related behaviors in rodents via spatiotemporal polarity dynamics tuning.Nature communications · 2026Article
- An orthogonal TRAP enables intersectional genetic access to activated neurons in the mouse brain.bioRxiv : the preprint server for biology · 2026Article
- Optogenetic engineering of synthetic and natural receptors: design principles, functional mechanisms and biomedical applications.Regenerative biomaterials · 2026Review
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- A Bioluminescent Activity Dependent (BLADe) Platform for Converting Intracellular Activity to Photoreceptor Activation.bioRxiv : the preprint server for biology · 2025Article
- 2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neurons.bioRxiv : the preprint server for biology · 2025Article
- Synergistic Reinforcement Learning by Cooperation of the Cerebellum and Basal Ganglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Cutting-edge methodologies for tagging and tracing active neuronal coding in the brain.Current opinion in neurobiology · 2025Review
- Corticothalamic neurons in motor cortex have a permissive role in motor execution.Nature communications · 2025Article
- Protein Engineering for Spatiotemporally Resolved Cellular Monitoring.Annual review of analytical chemistry (Palo Alto, Calif.) · 2025Review
- An improved FLARE system for recording and manipulating neuronal activity.Cell reports methods · 2025Article
- Principles and Design of Molecular Tools for Sensing and Perturbing Cell Surface Receptor Activity.Chemical reviews · 2025Review
34 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite recent advances in optogenetics, it remains challenging to manipulate gene expression in specific populations of neurons. We present a dual-protein switch system, Cal-Light, that translates neuronal-activity-mediated calcium signaling into gene expression in a light-dependent manner. In cultured neurons and brain slices, we show that Cal-Light drives expression of the reporter EGFP with high spatiotemporal resolution only in the presence of both blue light and calcium. Delivery of the Cal-Light components to the motor cortex of mice by viral vectors labels a subset of excitatory and inhibitory neurons related to learned lever-pressing behavior. By using Cal-Light to drive expression of the inhibitory receptor halorhodopsin (eNpHR), which responds to yellow light, we temporarily inhibit the lever-pressing behavior, confirming that the labeled neurons mediate the behavior. Thus, Cal-Light enables dissection of neural circuits underlying complex mammalian behaviors with high spatiotemporal precision.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.