ReviewCurrent opinion in structural biology2017
Taking the measure of MicroED.
Review in Current opinion in structural biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- An Amino-Thiophene Functionalized Metal-Organic Framework on Fabric for Selective Extraction, Recovery, and Passive Sampling of Gold Ions and Nanoparticles.Chemistry of materials : a publication of the American Chemical Society · 2025Article
- Fast event-based electron counting for small-molecule structure determination by MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- 3D Nanocrystallography and the Imperfect Molecular Lattice.Annual review of physical chemistry · 2024Review
- Forty years in cryoEM of membrane proteins.Microscopy (Oxford, England) · 2022Article
- Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation.Acta crystallographica. Section D, Structural biology · 2021Article
- Taming Radical Pairs in the Crystalline Solid State: Discovery and Total Synthesis of Psychotriadine.Journal of the American Chemical Society · 2021Article
- Amyloid Oligomers: A Joint Experimental/Computational Perspective on Alzheimer's Disease, Parkinson's Disease, Type II Diabetes, and Amyotrophic Lateral Sclerosis.Chemical reviews · 2021Review
- Ab Initio Determination of Peptide Structures by MicroED.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The complementarity of serial femtosecond crystallography and MicroED for structure determination from microcrystals.Current opinion in structural biology · 2019Review
- Data-driven challenges and opportunities in crystallography.Emerging topics in life sciences · 2019Article
- The cryo-EM method microcrystal electron diffraction (MicroED).Nature methods · 2019Review
- Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ.IUCrJ · 2019Article
- Article
- Nanoscale mosaicity revealed in peptide microcrystals by scanning electron nanodiffraction.Communications biology · 2019Article
- MicroED structures of HIV-1 Gag CTD-SP1 reveal binding interactions with the maturation inhibitor bevirimat.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- MicroED: a versatile cryoEM method for structure determination.Emerging topics in life sciences · 2018Article
- Where is crystallography going?Acta crystallographica. Section D, Structural biology · 2018Review
- Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp.Nature structural & molecular biology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
It is now possible to routinely determine atomic resolution structures by electron cryo-microscopy (cryoEM), facilitated in part by the method known as micro electron-diffraction (MicroED). Since its initial demonstration in 2013, MicroED has helped determine a variety of protein structures ranging in molecular weight from a few hundred Daltons to several hundred thousand Daltons. Some of these structures were novel while others were previously known. The resolutions of structures obtained thus far by MicroED range from 3.2Å to 1.0Å, with most better than 2.5Å. Crystals of various sizes and shapes, with different space group symmetries, and with a range of solvent content have all been studied by MicroED. The wide range of crystals explored to date presents the community with a landscape of opportunity for structure determination from nano crystals. Here we summarize the lessons we have learned during the first few years of MicroED, and from our attempts at the first ab initio structure determined by the method. We re-evaluate theoretical considerations in choosing the appropriate crystals for MicroED and for extracting the most meaning out of measured data. With more laboratories worldwide adopting the technique, we speculate what the first decade might hold for MicroED.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.