ArticleAnalytical chemistry2017
A Printed Equilibrium Dialysis Device with Integrated Membranes for Improved Binding Affinity Measurements.
Article in Analytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Molecular determinants of cholesterol binding to BK channel-forming slo1 subunits.Archives of biochemistry and biophysics · 2026Article
- Toward a Disruptive "Click-and-Run" 3D Printing Concept for Manufacturing Epidermal Wearable Electrochemical Sensors.ACS sensors · 2025Review
- 3D printed microfluidic devices with electrodes for electrochemical analysis.Analytical methods : advancing methods and applications · 2024Review
- Functionality integration in stereolithography 3D printed microfluidics using a "print-pause-print" strategy.Lab on a chip · 2024Article
- Simple Determination of Affinity Constants of Antibodies by Competitive Immunoassays.Methods and protocols · 2024Article
- A 3D-printed multi-compartment device that enables dynamic PK/PD profiles of antibiotics.Analytical and bioanalytical chemistry · 2023Article
- A 3D-printed multi-compartment device that enables dynamic PK/PD profiles of antibiotics.Analytical and bioanalytical chemistry · 2023Article
- Tunable resins with PDMS-like elastic modulus for stereolithographic 3D-printing of multimaterial microfluidic actuators.Lab on a chip · 2023Article
- Addendum: A 'print-pause-print' protocol for 3D printing microfluidics using multimaterial stereolithography.Nature protocols · 2023Article
- A novel 3D-printed centrifugal ultrafiltration method reveals in vivo glycation of human serum albumin decreases its binding affinity for zinc.Metallomics : integrated biometal science · 2020Article
- Powder-Based 3D Printing for the Fabrication of Device with Micro and Mesoscale Features.Micromachines · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Equilibrium dialysis is a simple and effective technique used for investigating the binding of small molecules and ions to proteins. A three-dimensional (3D) printer was used to create a device capable of measuring binding constants between a protein and a small ion based on equilibrium dialysis. Specifically, the technology described here enables the user to customize an equilibrium dialysis device to fit their own experiments by choosing membranes of various material and molecular-weight cutoff values. The device has dimensions similar to that of a standard 96-well plate, thus being amenable to automated sample handlers and multichannel pipettes. The device consists of a printed base that hosts multiple windows containing a porous regenerated-cellulose membrane with a molecular-weight cutoff of ∼3500 Da. A key step in the fabrication process is a print-pause-print approach for integrating membranes directly into the windows subsequently inserted into the base. The integrated membranes display no leaking upon placement into the base. After characterizing the system's requirements for reaching equilibrium, the device was used to successfully measure an equilibrium dissociation constant for Zn
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.