Evidence map›Paper›PMID 28647785›Full record

ArticleJournal of assisted reproduction and genetics2017

Fertility preservation for trans men: frozen-thawed in vitro matured oocytes collected at the time of ovarian tissue processing exhibit normal meiotic spindles.

S Lierman, K Tilleman, K Braeckmans, K Peynshaert, S Weyers, G T'Sjoen, P De Sutter

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
5.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 82 citations in OpenAlex.

  1. Review
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  7. New Insights on In Vitro Maturation of Oocytes for Fertility Preservation.International journal of molecular sciences · 2024
    Review
  8. Article
  9. Review
  10. Reproductive capacity after gender-affirming testosterone therapy.Human reproduction (Oxford, England) · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

S LiermanDepartment for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium. sylvie.lierman@UGent.be.
K TillemanDepartment for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.
K BraeckmansDepartment of Pharmaceutics, Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
K PeynshaertDepartment of Pharmaceutics, Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Harelbekestraat 72, 9000, Ghent, Belgium.
S WeyersDepartment of Gynaecology, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.
G T'SjoenDepartment of Endocrinology and Centre for Sexuoloy and Gender, De Pintelaan 185, 9000, Ghent, Belgium.
P De SutterDepartment for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.
Ghent University Hospital · BEGhent University · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposesAt the moment of sex reassignment surgery (SRS), the ovarian tissue is sometimes cryopreserved as fertility preservation option for female-to-male trans men, also called trans men. During this preparation, cumulus-oocyte-complexes (COCs) can be found and in vitro matured. It is not known if these oocytes are developmentally competent. In order to use these oocytes for fertility preservation and subsequent fertilization, a normal spindle structure before and after vitrification is necessary.

methodsA total of 680 COCs were collected from trans men (n = 16) at the time of SRS and after testosterone treatment. The COCs were subjected to in vitro maturation and those that reached the metaphase II stage (MII) were collected and split into two groups; group 1 was immediately fixed for spindle staining and group 2 was first vitrified and warmed followed by spindle staining. Statistical analysis was performed by Fisher's exact test.

resultsAfter 48 h in vitro maturation, 38.1% of COCs were at MII stage. Those oocytes were split in two groups: (1) 126 MII oocytes in the noncryopreservation group and (2) 133 MII oocytes underwent cryopreservation through vitrification. The oocyte survival rate, after 2 h warming, was 67.7%. Both the noncryopreserved and the vitrified group showed comparable results concerning normal spindle structure and chromosomes alignment, 85.7% vs. 92.2% (P = 0.27).

conclusionsSpindle structure analysis and chromosomal alignment after vitrification seem normal in in vitro matured COCs collected during the tissue processing of ovaries in trans men at the time of SRS. The MII oocytes do not seem to be morphologically affected by prolonged testosterone treatment.

Indexed as

AdultCryopreservationFemaleFertility PreservationGender-Affirming SurgeryHumansIn Vitro Oocyte Maturation TechniquesMaleOocytesOvarySpindle ApparatusTransgender PersonsVitrificationHuman ovarian medulla tissueIn vitro maturationMetaphase II oocytesSpindle structureVitrification

Identifiers

PMID28647785
PMCPMC5700000
OpenAlexW2632602878

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.