ArticleBiophysical journal2017
Vesicle Fusion Mediated by Solanesol-Anchored DNA.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Haptotactic Motion of Multivalent Vesicles Along Ligand-Density Gradients.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Article
- QCM-D Investigations on Cholesterol-DNA Tethering of Liposomes to Microbubbles for Therapy.The journal of physical chemistry. B · 2023Article
- Membrane fusion studied by colloidal probes.European biophysics journal : EBJ · 2021Review
- Hydrophobic Interaction: A Promising Driving Force for the Biomedical Applications of Nucleic Acids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Controlled Peptide-Mediated Vesicle Fusion Assessed by Simultaneous Dual-Colour Time-Lapsed Fluorescence Microscopy.Scientific reports · 2020Article
- Barcoding Biological Reactions with DNA-Functionalized Vesicles.Angewandte Chemie (International ed. in English) · 2019Review
- The Fusion of Lipid and DNA Nanotechnology.Genes · 2019Review
- Bioactivities and Medicinal Value of Solanesol and Its Accumulation, Extraction Technology, and Determination Methods.Biomolecules · 2019Review
- Interaction of SNARE Mimetic Peptides with Lipid bilayers: Effects of Secondary Structure, Bilayer Composition and Lipid Anchoring.Scientific reports · 2019Article
- Dynamic Interactions between Lipid-Tethered DNA and Phospholipid Membranes.Langmuir : the ACS journal of surfaces and colloids · 2018Article
- Peptide-Mediated Liposome Fusion: The Effect of Anchor Positioning.International journal of molecular sciences · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Fusion between two lipid bilayers is one of the central processes in cell biology, playing a key role in endocytosis, exocytosis, and vesicle transport. We have previously developed a model system that uses the hybridization of complementary DNA strands to model the formation of the SNARE four-helix bundle that mediates synaptic vesicle fusion and used it to study vesicle fusion to a tethered lipid bilayer. Using single vesicle assays, 70% of observed fusion events in the DNA-lipid system are arrested at the hemifusion stage, whereas only 5% eventually go to full fusion. This may be because the diglycerol ether that anchors the DNA in the membrane spans only half the bilayer: upon hemifusion and mixing of the outer leaflets, the DNA-lipid is free to diffuse into the target membrane and away from the vesicle. Here, we test the hypothesis that the length of the membrane anchor may impact the outcome by comparing single leaflet-spanning DNA-lipid mediated vesicle fusion with fusion mediated by DNA anchored by solanesol, a C45 isoprenoid of sufficient length to span the bilayer. When the solanesol anchor was present on the incoming vesicles, target membrane, or both, ∼2-3 times as much full fusion was observed as in the DNA-lipid mediated system, as measured by lipid mixing or content transfer. These results indicate that a transmembrane anchor increases the efficiency of full fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.